Anti-amoebic activity of a cecropin-melittin hybrid peptide (CM11) against trophozoites of Entamoeba histolytica
Anti-amoebic activity of a cecropin-melittin hybrid peptide (CM11) against trophozoites of Entamoeba histolytica
复制标题
DOI：
10.1007/s00508-019-01540-9
复制
发表时间：
2019-09-01
影响因子：
2.6
通讯作者：
Dalimi, Abdolhossein
中科院分区：
文献类型：
--
作者：
Abhari, Fatemeh Mahdavi;Pirestani, Majid;Dalimi, Abdolhossein
溶组织内阿米巴是一种肠道寄生虫，位于人体肠腔内，可以攻击上皮细胞。抗菌肽 (AMP) 可有效对抗多种微生物，如细菌、真菌、病毒、酵母菌和原生动物。 CM11 是一种嵌合肽，源自蜂毒和蝴蝶化合物。在本研究中，在不同浓度的肽和甲硝唑中测定了 CM11 对人结肠癌 (Caco-2) 细胞和溶组织内阿米巴的细胞毒性作用。 MTT结果显示，24&x202f;mu g/ml的CM11肽在24&x202f;h和48&x202f;h时对Caco-2细胞的细胞毒性百分比最高，分别为49.8%和44.3%。甲硝唑组在24&x202f;h和48&x202f;h后观察到40&x202f;mu g/ml浓度的细胞毒性最高，分别为43.5%和42.1%。 CM11对Caco-2诱导的细胞凋亡率在24&x202f;h和48&x202f;h后最高分别为53.9%和51.4%；然而，甲硝唑组的这一比例分别为 19.1% 和 33.4%。肽和甲硝唑在 24 & x202f;h 和 48 & x202f;h 对溶组织内阿米巴的作用表明，在最高浓度的 CM11 肽 (24 & x202f;mu g/ml) 下，细胞毒性作用分别为 93.7% 和 94.9%，甲硝唑 (40 & x202f;mu g/ml) 的细胞毒性作用为 65.5% 和分别为 74.3%。在共培养中，最高浓度的CM11和甲硝唑分别杀死了63.5%和57.7%的寄生虫。本研究结果表明CM11肽对溶组织阿米巴具有较高的毒性，未来抗菌肽的使用可考虑作为抗阿米巴化合物。
Entamoeba histolytica is an intestinal parasite that is located in the lumen of the human intestine and can attack the epithelium. Antimicrobial peptides (AMPs) are effective against the wide range of microorganisms, such as bacteria, fungi, viruses, yeasts, and protozoa. The CM11 is a chimeric peptide that is derived from bee venom and butterfly compounds. In this study, the cytotoxic effect of CM11 on Human colonic carcinoma (Caco-2) cells and E. histolytica were assayed in various concentrations of peptide and metronidazole. The MTT results showed that the highest percentage of cytotoxicity on Caco-2 cells was in 24 & x202f;mu g/ml of CM11 peptide at 24 & x202f;h and 48 & x202f;h, which was 49.8%, and 44.3%, respectively. In the metronidazole group, the highest cytotoxicity with 40 & x202f;mu g/ml concentration was observed after 24 & x202f;h and 48 & x202f;h, with 43.5%, and 42.1%, respectively. The highest rate of apoptosis induced by CM11 on Caco-2 was 53.9% and 51.4% after 24 & x202f;h and 48 & x202f;h, respectively; however, these rates were 19.1% and 33.4% in the metronidazole group. The effect of peptide and metronidazole on E. histolytica at 24 & x202f;h and 48 & x202f;h showed that at the highest concentration of CM11 peptide (24 & x202f;mu g/ml) the cytotoxic effect was 93.7% and 94.9% and for metronidazole (40 & x202f;mu g/ml) was 65.5% and 74.3%, respectively. In coculture, 63.5% and 57.7% of parasites were killed in the highest concentration of CM11 and metronidazole, respectively. The results of this study revealed that CM11 peptide has a high toxicity on E. histolytica, and the use of antimicrobial peptides in the future can be considered as anti-amoebic compounds.