Effect of Faradic products on direct current-stimulated calvarial organ culture calcium levels
Effect of Faradic products on direct current-stimulated calvarial organ culture calcium levels
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DOI：
10.1006/bbrc.1999.1355
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发表时间：
1999-11-02
影响因子：
3.1
通讯作者：
Stevens, CR
中科院分区：
文献类型：
--
作者：
Bodamyali, T;Kanczler, JM;Stevens, CR
从小鼠颅骨器官培养物中释放的钙被用来分析恒定直流电（20 μ A）结合在钛丝阴极产生的法拉第产物的良好描述的生物学效应。恒定的20 μ A直流电刺激，单独交付的琼脂盐桥，一贯降低媒体钙水平。直接暴露的颅骨钛阴极及其感应产物导致进一步降低介质钙水平，也显着增加介质pH值。过氧化氢是一种感应产物的钛阴极，微摩尔量正在产生我们的系统超过24小时。H2O2是促吸收，而pH值升高刺激成骨细胞活性。我们认为，在骨组织与金属丝阴极直接接触的情况下，感应产物、过氧化氢和氢氧根离子是重要因素，其本身进一步促进了重建加速和临床结果改善。(C)北京：科学出版社.
Calcium release from mouse calvarial organ cultures was used to analyse the well-described biological effects of constant direct current (20 mu A) in combination with Faradic products generated at a titanium wire cathode. Constant 20-mu A direct current stimulation alone, delivered by agar salt bridges, consistently lowered the media calcium levels. Direct exposure of calvariae to a titanium cathode and its faradic products resulted in further lowering of media calcium levels and also a significant increase in the media pH. Hydrogen peroxide is a faradic product of the titanium cathode, micromolar amounts being generated by our system over 24 hr. H2O2 is pro-resorptive whereas elevated pH stimulates osteoblast activity. We propose that where bone tissue is in direct contact with metal wire cathodes, the faradic products, hydrogen peroxide and hydroxyl ion, are significant factors which, in their own right, further contribute to accelerated remodelling and improved clinical outcome. (C) 1999 Academic Press.