Time- and concentration-dependent effects of dissolution products of 58S sol-gel bioactive glass on proliferation and differentiation of murine and human osteoblasts
Time- and concentration-dependent effects of dissolution products of 58S sol-gel bioactive glass on proliferation and differentiation of murine and human osteoblasts
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DOI：
10.1089/1076327041887664
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Polak, JM
中科院分区：
文献类型：
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作者：
Bielby, RC;Christodoulou, IS;Polak, JM
骨丢失是一个重要的临床问题，利用捐赠的移植材料进行治疗是有限的。为了满足医疗保健行业的未来需求，人们的观点已经转向使用生物活性材料进行组织再生。许多体内和体外研究都强调了生物活性玻璃陶瓷45 S5 Bioglass作为合成再生支架的潜力。溶胶-凝胶工艺技术的应用导致了具有更大织构和组成多样性的介孔生物活性玻璃的合成。在这项研究中，我们评估了补充组织培养基中含有高达203 ppm的二氧化硅静态浸泡颗粒的58 S溶胶-凝胶生物活性玻璃（58%SiO2，33%CaO，9%P2O5）在体外增殖和分化的小鼠和人的原代成骨细胞。这些提取物的二氧化硅含量高于先前在45 S5生物玻璃研究中使用的那些，因为介孔玻璃的更高表面积与体积比允许更快的离子交换速率。我们发现，成骨细胞从这两个物种增加了他们的增殖反应的玻璃条件培养基。此外，补充培养基可以改变细胞分化的程度随培养时间而变化。由补充培养基诱导的增殖抑制由碱性成纤维细胞生长因子（一种已知的成骨细胞促有丝分裂生长因子）处理诱导的效应。骨结节的形成也增加了暴露于玻璃条件培养基，这种效果与用于制备培养基的玻璃剂量呈正相关。玻璃条件培养液可刺激小鼠成骨细胞凋亡，但抑制人成骨细胞凋亡。这些数据证明了源自溶胶-凝胶材料的溶解产物对原代成骨细胞的生物活性作用，并补充了体内研究，表明该材料适合作为骨移植替代品。
Bone loss is a significant clinical problem, and treatments utilizing donated graft material are limited. To meet future demands in the healthcare industry, there has been a shift of outlook toward the use of bioactive materials for tissue regeneration. A number of in vivo and in vitro studies have highlighted the potential of the bioactive glass ceramic 45S5 Bioglass as a synthetic regenerative scaffold. The application of sol-gel processing techniques has led to the synthesis of mesoporous bioactive glasses with greater textural and compositional variety. In this study, we evaluated the effects of supplemented tissue culture medium containing up to 203 ppm silica prepared by static soaking of particles of 58S sol-gel bioactive glass (58% SiO2, 33% CaO, 9% P2O5) on the in vitro proliferation and differentiation of murine and human primary osteoblasts. These extracts had a higher silica content than those used previously in studies of 45S5 Bioglass, because of the faster rates of ion exchange permitted by the higher surface area-to-volume ratio of mesoporous glass. We found that osteoblasts from both species increased their proliferation in response to the glass-conditioned medium. In addition, the extent to which supplemented medium could alter cell differentiation varied with time in culture. Proliferation induced by supplemented medium paralleled effects induced by treatment with basic fibroblast growth factor, a known mitogenic growth factor for osteoblasts. Bone nodule formation was also increased by exposure to the glass-conditioned medium and this effect was positively correlated with the dose of glass used to prepare the medium. Apoptosis was stimulated by glass-conditioned medium in murine osteoblasts, but inhibited in human osteoblasts. These data demonstrate the bioactive effects of dissolution products derived from sol-gel materials on primary osteoblasts and complements in vivo studies that indicate the suitability of this material as a bone graft substitute.