Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis
Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis
复制标题
DOI：
10.1111/jdv.12753
复制
发表时间：
2015-06-01
影响因子：
9.2
通讯作者：
Aoki, V.
中科院分区：
文献类型：
--
作者：
Batista, D. I. S.;Perez, L.;Aoki, V.
研究背景成人特应性皮炎（AD）与皮肤屏障蛋白和炎症细胞因子的关系。研究目的：探讨AD患者皮肤屏障蛋白如丝聚蛋白、密蛋白1和4以及外周血炎症细胞因子（Th 1/Th 2/Th 17）的表达。AD的严重程度用兴奋面积和严重程度指数（EASI）来衡量。实验室分析包括皮肤屏障蛋白的免疫组织化学分析，例如来自皮肤样品的聚丝蛋白、密蛋白1和4以及白细胞介素-17（IL-17），以及循环细胞因子水平的测定（IL-2，4，5，6，10，17 A，TNF和IFN-α），通过流式细胞术结果与对照组相比，我们观察到AD患者皮损皮肤中丝聚蛋白和密蛋白1的表达减少。丝聚蛋白表达与疾病严重程度呈负相关。此外，IL-17表达在AD患者中增强。同样，较高水平的炎症细胞因子（IL-2，5，6，10，17 A和IFN-）被发现在AD patients.ConclusionOur数据加强了AD的发病机制中的作用，改变皮肤屏障。我们的研究结果表明，不仅减少表达的聚丝蛋白和claudin 1在病变特应性皮肤，但聚丝蛋白的表达和疾病的严重程度呈负相关。此外，原位IL-17和循环中的白细胞介素水平在AD中的升高强调了这种缺陷性皮肤屏障皮肤病的全身性炎症特征。
BackgroundAtopic dermatitis (AD) in adults and profile of skin barrier proteins and inflammatory cytokines.ObjectiveEvaluation of the expression of skin barrier proteins such as filaggrin, claudins 1 and 4 and of circulating inflammatory cytokines (Th1/Th2/Th17) in adults with AD.MethodsThirty-three adult patients with AD diagnosed according to the Hanifin & Rajkacriteria, and 25 healthy controls were enrolled in the study. AD severity was measured by Eczema Area and Severity Index (EASI). Laboratory assays included immunohistochemistry analysis of skin barrier proteins, such as filaggrin, claudins 1 and 4 and interleukin-17 (IL-17) from skin samples and determination of circulating cytokine levels (IL-2, 4, 5, 6, 10, 17A, TNF and IFN-) by flow cytometry (Cytometric Bead Array).ResultsWe observed a reduced expression of filaggrin and claudin 1 in lesional skin of AD patients, when compared to controls. There was an inverse correlation of filaggrin expression and disease severity. In addition, IL-17 expression was enhanced in AD patients. Similarly, higher levels of inflammatory cytokines (IL-2, 5, 6, 10, 17A and IFN-) were found in AD patients.ConclusionOur data reinforce the role of an altered skin barrier in the pathogenesis of AD. Our results show not only reduced expression of filaggrin and claudin 1 in lesional atopic skin but also inverse correlation of filaggrin expression and disease severity. Moreover, elevation of in situ IL-17 and of circulating interleukin levels in AD emphasize the systemic, inflammatory profile of this defective skin barrier dermatosis.