Acyl and alkyl chain length of GPI-anchors is critical for raft association in vitro
Acyl and alkyl chain length of GPI-anchors is critical for raft association in vitro
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DOI：
10.1016/s0014-5793(99)01501-x
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发表时间：
1999-11-26
期刊：
影响因子：
3.5
通讯作者：
Simons, K
中科院分区：
文献类型：
--
作者：
Benting, J;Rietveld, A;Simons, K
我们确定了从MDCK和Fischer大鼠甲状腺（FRT）细胞中分离的GPI锚定物的酰基和烷基链组成。两种细胞系都合成含有C16/C18或C18/C18饱和酰基和烷基链的GPI锚。GPI锚定的胎盘碱性磷酸酶（PLAP）在这两种细胞中表达的筏相关和FLAP从FRT细胞纯化筏相关的体外重组成脂质体含有筏脂质。与此相反，GPI锚定的变体表面糖蛋白从布氏锥虫，其中包含C14酰基和烷基链，在体外重建后没有显示出显着的筏协会。这些数据表明，GPI锚的酰基和烷基链组成决定筏协会。(C)1999年欧洲生物化学学会联合会。
We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and FLAP purified from FRT cells is raft-associated in vitro when reconstituted into liposomes containing raft lipids. In contrast, the GPI-anchored variant surface glycoprotein from Trypanosoma brucei which contains C14 acyl and alkyl chains shows no significant raft association after reconstitution in vitro. These data indicate that the acyl and alkyl chain composition of GPI-anchors determines raft association. (C) 1999 Federation of European Biochemical Societies.