The ribonucleases J1 and J2 are essential for growth and have independent roles in mRNA decay in Streptococcus pyogenes
The ribonucleases J1 and J2 are essential for growth and have independent roles in mRNA decay in Streptococcus pyogenes
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DOI：
10.1111/j.1365-2958.2009.07012.x
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发表时间：
2010-02-01
影响因子：
3.6
通讯作者：
Scott, June R.
中科院分区：
文献类型：
--
作者：
Bugrysheva, Julia V.;Scott, June R.
最近在枯草芽孢杆菌中鉴定出的旁系同源核糖核酸酶J1和J2具有内切核糖核酸酶和5 '至3'外切核糖核酸酶活性，并参与mRNA的降解和调节加工。RNase J1和J2具有部分重叠的靶特异性，但只有RNase J1对B是必需的。枯草杆菌生长由于mRNA衰变是重要的调节化脓性链球菌（A组链球菌，GAS）的毒力因子，我们研究了这些新描述的RNA酶在GAS中的作用。我们发现RNase J1和J2的条件突变体都需要诱导才能生长，因此我们得出结论，与B的情况不同。在枯草芽孢杆菌中，这两种RNA酶都是GAS生长所必需的，因此它们的功能不是冗余的。我们比较了我们之前确定的两类消息的代表的衰减：第一类，在增长的指数期和稳定期迅速衰减（hasA和gyrA），第二类，在稳定期稳定，并在指数期表现出双相衰减曲线（sagA和sda）。我们报告说，RNase J1和J2影响I类消息的衰减速率和II类消息衰减的第一阶段的长度。
The paralogous ribonucleases J1 and J2, recently identified in Bacillus subtilis, have both endoribonucleolytic and 5'-to-3' exoribonucleolytic activities and participate in degradation and regulatory processing of mRNA. RNases J1 and J2 have partially overlapping target specificities, but only RNase J1 is essential for B. subtilis growth. Because mRNA decay is important in regulation of virulence factors of Streptococcus pyogenes (the group A streptococcus, GAS), we investigated the role of these newly described RNases in GAS. We found that conditional mutants for both RNases J1 and J2 require induction for growth, so we conclude that, unlike the case in B. subtilis, both of these RNases are essential for GAS growth, and therefore their functions are not redundant. We compared decay of representatives of the two classes of messages we had previously identified: Class I, which decay rapidly in exponential and stationary phase of growth (hasA and gyrA), and Class II, which are stable in stationary phase and exhibit a biphasic decay curve in exponential phase (sagA and sda). We report that RNases J1 and J2 affect the rate of decay of Class I messages and the length of the first phase in decay of Class II messages.