Generation of fertile transplastomic soybean
Generation of fertile transplastomic soybean
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DOI：
10.1007/s11103-004-0192-4
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发表时间：
2004-07-01
影响因子：
5.1
通讯作者：
Tissot, G
中科院分区：
文献类型：
--
作者：
Dufourmantel, N;Pelissier, B;Tissot, G
我们在这里描述了大豆的质体转化方法的发展，大豆是一种重要的农艺兴趣豆科植物。用轰击法成功地转化了甘氨酸胚性组织的叶绿体。转化DNA携带一个受烟草质体调控表达元件控制的大观霉素抗性基因（aadA），两侧有两个相邻的大豆质体序列，可将其靶向插入trnV基因和rps12/7操纵子之间。所有产生的大观霉素抗性植株均为转质体，未检测到野生型质体副本。未获得自发突变体。转化效率与烟草质体相近。所有的转质体T0植株都是可育的，T1后代都具有一致的大霉素抗性，显示了质体转基因的稳定性。这是关于可育转质体大豆的首次报道。
We describe here the development of a plastid transformation method for soybean, a leguminous plant of major agronomic interest. Chloroplasts from embryogenic tissue of Glycine max have been successfully transformed by bombardment. The transforming DNA carries a spectinomycin resistance gene (aadA) under the control of tobacco plastid regulatory expression elements, flanked by two adjacent soybean plastome sequences allowing its targeted insertion between the trnV gene and the rps12/7 operon. All generated spectinomycin resistant plants were transplastomic and no remaining wild type plastome copies were detected. No spontaneous mutants were obtained. The transformation efficiency is similar to that of tobacco plastids. All transplastomic T0 plants were fertile and T1 progeny was uniformly spectinomycin resistant, showing the stability of the plastid transgene. This is the first report on the generation of fertile transplastomic soybean.