The nature of the Golgi apparatus. II. Identification of the Golgi apparatus with a complex of myelin figures
The nature of the Golgi apparatus. II. Identification of the Golgi apparatus with a complex of myelin figures
复制标题
高尔基体的性质。
DOI：
10.1002/jmor.1050850104
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发表时间：
1949
影响因子：
1.5
通讯作者：
A. Claude
中科院分区：
文献类型：
--
作者：
G. Palade;A. Claude
在先前的论文（Palade和Claude，'49）中，已经证明髓磷脂样结构可以通过乙醇在多种脊椎动物体细胞中诱导。已经发现这些数字表现出的形态和细胞内分布非常类似于那些在经典的细胞学文献中归因于相应细胞的高尔基体。这些观察结果表明，高尔基体可能是细胞标本制备过程中人工产生的细胞内髓鞘数字的集合。为了检验这一假说，有必要查明当按照展示高尔基体的推荐程序处理时，髓鞘样结构是否在细胞材料中发育。通常，这些方法包括两个主要的、连续的阶段：（a）固定，为此已经提出了各种水性固定剂混合物，和（B）浸渍，其通过四氧化锇或硝酸银溶液进行。然而，在这样的技术中，除了在浸渍之后，乙醇不介入。由于此时该装置已经存在于细胞中（Nassonov，'23），
I n a preceding paper (Palade and Claude, '49) it has been demonstrated that myelin figures can be induced by means of ethanol in a variety of vertebrate somatic cells. Such figures have been found to exhibit a morphology and an intracellular distribution closely similar to those ascribed in the classical cytological literature to the Golgi apparatus of the corresponding cells. These observations suggest that the Golgi apparatus might be a collection of intracellular myelin figures artificially produced during the preparation of the cytological specimens. I n order to test this hypothesis it is necessary to find out whether myelin figures develop in celltilar material while being treated according to the procedures recommended for the demonstration of the Golgi apparatus. Generally, these procedures comprise two main, successive stages: ( a ) the fixation, fo r which a variety of aqueous fixative mixtures have been proposed, and (b) the impregnation which is performed by means of osmium tetroxide, or silver nitrate solutions. I n such techniques, however, ethanol does not intervene except after impregnation. Since at that time the apparatus is already present in the cells (Nassonov, '23),