A Culture Device Demonstrates that Hydrostatic Pressure Increases mRNA of RGS5 in Neuroblastoma and CHC1-L in Lymphocytic Cells
A Culture Device Demonstrates that Hydrostatic Pressure Increases mRNA of RGS5 in Neuroblastoma and CHC1-L in Lymphocytic Cells
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DOI：
10.1159/000072718
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发表时间：
2003-09
影响因子：
2.7
通讯作者：
Y. Manome;N. Saeki;H. Yoshinaga;Michiko Watanabe;S. Mizuno
中科院分区：
文献类型：
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作者：
Y. Manome;N. Saeki;H. Yoshinaga;Michiko Watanabe;S. Mizuno
先前的研究表明，机械力影响广泛的细胞行为。这些力调节人体中重要的细胞反应，包括重力、静水压力、拉伸和剪切应力，这些力通过血流的通过施加在血管系统上。我们推断，这些力量可能是人体内细胞功能的重要和动态调节器。虽然已经研究了拉伸和剪切应力对细胞的影响，特别是对内皮细胞的影响，但对重力和静水压力对细胞的影响知之甚少。为了检查流体静压的直接影响，我们开发了一种培养装置，以赋予细胞0至1,000 psi的流体静压。我们进行了人神经母细胞瘤细胞和rIL-2激活的淋巴细胞，以恒定的压力为20或100 psi的48小时，并试图确定由静水压力调控的基因。静水压暴露后，神经母细胞瘤细胞中G蛋白信号转导调节因子5和淋巴细胞中CHC 1-L的基因增加。结果表明，静水压力直接调节哺乳动物细胞中特定基因的表达。此外，可能有一些潜在的机制在改变的物理环境中具有共同的影响。我们的体外培养系统可以提供一些深入的机制，通过细胞内的过程受到机械力的影响。
Previous studies demonstrated that mechanical forces affect a wide range of cellular behaviors. These forces regulate important cellular responses in the human body and consist of gravity, hydrostatic pressure, stretch, and shear stress, which is exerted on the vascular system by the passage of blood flow. We reasoned that these forces might be significant and dynamic regulators of cellular functions within the human body. While cellular effects of stretch and shear stress have been studied particularly with endothelial cells, little is known about the effects of gravity and hydrostatic pressure to cells. To examine the direct effect of hydrostatic pressure, we developed a culture device to confer hydrostatic pressures to cells ranging from 0 to 1,000 psi. We subjected human neuroblastoma cells and rIL-2-activated lymphocytes to a constant pressure of 20 or 100 psi for 48 h and attempted to identify genes regulated by hydrostatic pressure. Genes of regulator of G-protein signaling 5 in neuroblastoma cells and CHC1-L in lymphocytes increased after exposure to hydrostatic pressure. The results demonstrated that hydrostatic pressure directly regulates the expression of specific genes in mammalian cells. Moreover, there may be some underlying mechanisms that have common effects in altered physical environments. Our in vitro culture system may provide some insight into the mechanisms through the intracellular processes affected by mechanical forces.