EFFECTS OF OUABAIN AND OSMOLARITY ON BUMETANIDE-SENSITIVE POTASSIUM-TRANSPORT IN SIMIAN VIRUS-TRANSFORMED 3T3 CELLS
EFFECTS OF OUABAIN AND OSMOLARITY ON BUMETANIDE-SENSITIVE POTASSIUM-TRANSPORT IN SIMIAN VIRUS-TRANSFORMED 3T3 CELLS
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DOI：
10.1016/0005-2736(82)90564-8
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
LAMB, JF
中科院分区：
文献类型：
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作者：
BAKKERGRUNWALD, T;OGDEN, P;LAMB, JF
将猴病毒转化的小鼠成纤维细胞3 T3细胞中的单向K+内流分解为哇巴因可降解的泵组分、布美他尼敏感的Cl-依赖性共转运组分和残余漏通量。通过与哇巴因预孵育60分钟，布美他尼敏感组分被刺激2-3倍。随后洗涤细胞并在不含哇巴因的盐水中孵育，逆转了Na+泵的抑制和布美他尼敏感通量的刺激。高渗细胞收缩（由0.1 M或0.2 M山梨醇诱导）也刺激了布美他尼敏感性K+内流。布美他尼敏感系统可能在细胞体积调节中发挥作用。
Unidirectional K+ influx in simian virus-transformed mouse fibroblast 3T3 cells was dissected into a ouabain-inhibitable pump component, a bumetanide-sensitive and Cl-dependent cotransport component and a residual leak flux. The bumetanide-sensitive component was stimulated 2-3-fold by a 60-min preincubation with ouabain. Subsequent washing of the cells and incubation in ouabain-free saline reversed both the inhibition of the Na+ pump and the stimulation of bumetanide-sensitive flux. Bumetanide-sensitive K+ influx was also stimulated by hypertonic cell shrinkage (induced by 0.1 M or 0.2 M sorbitol). The bumetanide-sensitive system may play a role in cellular volume regulation.