Binding of tissue-plasminogen activator to fibrin: Effect of ultrasound
Binding of tissue-plasminogen activator to fibrin: Effect of ultrasound
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DOI：
10.1182/blood.v91.6.2019.2019_2019_2025
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发表时间：
1998-03-15
期刊：
影响因子：
20.3
通讯作者：
Francis, CW
中科院分区：
文献类型：
--
作者：
Siddiqi, F;Odrljin, TM;Francis, CW
Ultrasound reversibly alters the structure of polymerized fibrin, an effect that could influence tissue-plasminogen activator (t-PA) binding. We have, therefore, characterized the effects of ultrasound on binding of t-PA to fibrin using a novel system in which radiolabeled, active-site blocked, single chain tissue-plasminogen activator flowed through a fibrin gel at constant rate, and specific binding was determined by monitoring incorporation of radiolabel. Results using polymerized fibrin were compared with those using a surface of fibrin immobilized on Sepharose beads in a similar system. Interaction of t-PA with surface-immobilized fibrin involved two classes of binding sites (Kd = 31 nmol/L and 244 nmol/L) and a maximum binding ratio of 3.8 mol t-PA/mol fibrin. Ultrasound increased Kd for the high affinity site to 46 nmol/L (P
