The anti-apoptotic protein lifeguard is expressed in breast cancer cells and tissues
The anti-apoptotic protein lifeguard is expressed in breast cancer cells and tissues
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DOI：
10.2478/s11658-010-0009-1
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发表时间：
2010-06-01
影响因子：
8.3
通讯作者：
Vogt, Peter M.
中科院分区：
文献类型：
--
作者：
Bucan, Vesna;Reimers, Kerstin;Vogt, Peter M.
救生员（LFG）是一种抗凋亡蛋白，可抑制Fas介导的肿瘤细胞死亡。然而，人LFG在人乳腺细胞癌变中的分子功能尚不确定。我们研究了内源性LFG在四种乳腺癌细胞系（MCF-7，MDA-MB-231，T-47 D和HS 578 T），人乳腺上皮细胞系（HS 578 Bst），以及健康和癌性乳腺组织中的表达和功能。采用分子生物学技术（Western blot和RT-PCR）和免疫组化技术检测LFG的表达。为了研究Fas存在下乳腺癌细胞增殖，我们进行了荧光细胞活力测定。Fas与LFG的可能协会进行了分析，通过免疫荧光显微镜。在本文中，我们提供了令人信服的证据表明，LFG在几个人乳腺癌细胞系中过表达。更重要的是，我们发现LFG表达与原发性乳腺肿瘤的高肿瘤分级相关。最后，我们证明，Fas敏感性降低表达LFG的乳腺癌细胞系。我们的结果表明，LFG在乳腺癌上皮细胞中强烈表达。此外，LFG的过度表达与肿瘤分级和Fas敏感性降低相关。我们的研究结果支持了LFG可能在乳腺癌细胞凋亡下调中发挥作用的观点。
Lifeguard (LFG) is an anti-apoptotic protein that inhibits Fas-mediated death in tumour cells. However, the molecular function of human LFG in the carcinogenesis of human breast cells is uncertain. We studied the expression and function of endogenous LFG in four breast cancer cell lines (MCF-7, MDA-MB-231, T-47D and HS 578T), a human breast epithelial cell line (HS 578Bst), and in healthy and cancerous breast tissues. Molecular (Western blot and RT-PCR) and immunohistochemical techniques were used to investigate the LFG expression. To investigate the breast cancer cell proliferation in the presence of Fas, we performed fluorescent cell viability assays. The possible association of Fas with LFG was analyzed by immunofluorescence microscopy. In this paper, we provide convincing evidence that LFG is overexpressed in several human breast cancer cell lines. More importantly, we found that the LFG expression correlates with high tumour grades in primary breast tumours. Finally, we demonstrated that Fas sensitivity is reduced in breast cancer cell lines expressing LFG. Our results indicated that LFG is strongly expressed in breast cancer epithelial cells. Moreover, the overexpression of LFG correlated with tumour grade and reduced Fas sensitivity. Our findings support the idea that LFG may have a role in the downregulation of apoptosis in breast cancer cells.