Effects of pond water, sediment and sediment extract samples from New Hampshire, USA on early Xenopus development and metamorphosis: Comparison to native species
Effects of pond water, sediment and sediment extract samples from New Hampshire, USA on early Xenopus development and metamorphosis: Comparison to native species
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DOI：
10.1002/jat.740
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发表时间：
2001-05-01
影响因子：
3.3
通讯作者：
Rolland, R
中科院分区：
文献类型：
--
作者：
Fort, DJ;Rogers, RL;Rolland, R
在努力评估潜在的生态危害，两栖动物物种在选定的地区在新南普郡，传统的青蛙胚胎致畸试验-非洲爪蟾（FETAX），14-/21天的尾巴吸收甲状腺破坏试验和> 30天的肢体发育测试进行了代表性的地表水和沉积物样品。对从五个地点收集的两组单独的样品进行了评价。本研究的主要目的是确定样品是否能够诱导早期胚胎-幼虫发育不良，确定发育不良是否包括肢体缺陷，确定甲状腺素联合给药是否改变肢体畸形的发生率，并评价样品对生长速率、发育进展和变态高潮的影响。这些研究的结果表明，池塘水和沉积物提取物样品，但不是整个沉积物样品，从B2，FW，LP和W池塘能够诱导异常早期胚胎-幼虫发育。此外，从B2和W池塘的水样诱导显着异常后肢发育。在尾部吸收研究中注意到一些异常前肢发育，但程度与后肢不同。每个水样均诱导明显的发育延迟，包括配对参考位点B1，其可通过添加外源性甲状腺素逆转，版权所有（C）2001 John Wiley & Sons，Ltd.
In an effort to assess potential ecological hazards to amphibian species in selected regions within New Nampshire, the traditional Frog Embryo Teratogenesis Assay-Xenopus (FETAX), a 14-/21 day tail resorption thyroid disruption assay and > 30 day limb development tests were conducted with representative surface water and sediment samples. Two separate sets of samples collected from five sites were evaluated. The primary objectives of the study were to determine if samples were capable of inducing early embryo-larval maldevelopment, to determine if maldevelopment included limb defects, to determine if thyroxine co-administration altered the rates of limb malformation and to evaluate the impact of the samples on growth rates, developmental progress and metamorphic climax. Results from these studies suggested that pond water and sediment extract samples, but not whole sediment samples, from B2, FW, LP and W ponds were capable of inducing abnormal early embryo-larval development. In addition, water samples from B2 and W ponds induced significant abnormal hindlimb development. Some abnormal forelimb development was noted in the tail resorption studies, but not to the same extent as the hindlimbs, Each of the water samples induced appreciable developmental delay, including the paired reference site B1, which could be reversed by the addition of exogenous thyroxine, Copyright (C) 2001 John Wiley & Sons, Ltd.