RESPONSES TO IRON-DEFICIENCY IN ARABIDOPSIS-THALIANA - THE TURBO IRON REDUCTASE DOES NOT DEPEND ON THE FORMATION OF ROOT HAIRS AND TRANSFER CELLS
RESPONSES TO IRON-DEFICIENCY IN ARABIDOPSIS-THALIANA - THE TURBO IRON REDUCTASE DOES NOT DEPEND ON THE FORMATION OF ROOT HAIRS AND TRANSFER CELLS
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DOI：
10.1007/bf00195707
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发表时间：
1995-02-01
期刊：
影响因子：
4.3
通讯作者：
KUIPER, PJC
中科院分区：
文献类型：
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作者：
MOOG, PR;VANDERKOOIJ, TAW;KUIPER, PJC
拟南芥菜(L.)海因。哥伦比亚野生型和根无毛突变体RM57在含铁和缺铁的营养液中生长。缺铁诱导完整根的铁络合还原酶(FCR)符合Michaelis-Menten动力学，野生型和突变体的K-m分别为45和54 mU，V-max分别为42和33nmolFe~(2+)·(-1)·min~(-1)。该反应的最适pH为5.5左右。FCR活性的大约4倍刺激与缺铁诱导的根毛和/或转移细胞的形成无关。缺铁诱导的黄化和硬根习性的形成在叶面施铁后消失，而FCR活性保持不变。对缺铁反应的时间进程表明，形态反应和生理反应分别受到控制。
Arabidopsis thaliana (L.) Heynh. Columbia wild type and a root hair-less mutant RM57 were grown on iron-containing and iron-deficient nutrient solutions. In both genotypes, ferric chelate reductase (FCR) of intact roots was induced upon iron deficiency and followed a Michaelis-Menten kinetic with a K-m of 45 and 54 mu M Fe-III-EDTA and a V-max of 42 and 33 nmol Fe2+.(g FW)(-1).min(-1) for the wild type and the mutant, respectively. The pH optimum for the reaction was around pH 5.5. The approximately four fold stimulation of FCR activity was independent of formation of root hairs and/or transfer cells induced by iron deficiency. Iron-deficiency-induced chlorosis and the development of a rigid root habit disappeared when ferric chelate was applied to the leaves, while FCR activity remained unchanged. The time course of the responses to iron deficiency showed that morphological and physiological responses were controlled separately.