Evidence for a chloride conductance in secretory membrane of parietal cells.
Evidence for a chloride conductance in secretory membrane of parietal cells.
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壁细胞分泌膜中氯离子电导的证据。
DOI：
10.1152/ajpgi.1989.256.2.g299
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hersey,SJ
中科院分区：
文献类型：
--
作者：
Perez,A;Blissard,D;Sachs,G;Hersey,SJ
采用吖啶橙作为探针的荧光猝灭方法用于监测去污剂渗透的胃腺原位酸的形成。在 KCl 培养基中，向透化腺体中添加 ATP 导致荧光快速下降，而添加缬氨霉素则导致第二阶段的荧光猝灭。通过添加 H+-K+-ATP 酶抑制剂 Sch 28080，荧光得以恢复。在 K2SO4 或 K+-羟乙基磺酸盐介质中也观察到 ATP 依赖性荧光猝灭；然而，缬氨霉素在不含 Cl 的培养基中无效。在 KCl 培养基中，ATP 依赖性猝灭可以被生电质子载体四氯水杨酰苯胺 (TCS) 逆转或阻止，但在无 Cl 培养基中则不然。 TCS 的结果被解释为证明分泌膜中存在较大的 Cl- 电导，而缬氨霉素的结果表明静息膜缺乏 K+ 电导。数据表明，可能表现出 Cl-电导的复杂 KCl 途径被用来激活酸分泌。
A fluorescence-quench method using acridine orange as the probe was employed to monitor acid formation in situ by detergent-permeabilized gastric glands. In KCl medium, the addition of ATP to the permeabilized glands resulted in a rapid decrease in fluorescence and addition of valinomycin resulted in a second phase of fluorescence quench. The fluorescence was restored by addition of the H+-K+-ATPase inhibitor, Sch 28080. An ATP-dependent fluorescence quench was observed also in K2SO4 or K+-isethionate medium; however, valinomycin was ineffective in the Cl-free media. The ATP-dependent quench could be reversed or prevented by the electrogenic protonophore, tetrachlorosalicylanilide (TCS), in KCl medium but not in Cl-free media. The results with TCS are interpreted as demonstrating a large Cl- conductance in the secretory membrane, whereas the results with valinomycin indicate that resting membranes lack a K+ conductance. The data suggest that a complex KCl pathway that may demonstrate a Cl- conductance is used to activate acid secretion.