Interferon but not MxB inhibits foamy retroviruses
Interferon but not MxB inhibits foamy retroviruses
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DOI：
10.1016/j.virol.2015.10.034
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发表时间：
2016-01-15
期刊：
影响因子：
3.7
通讯作者：
Muenk, Carsten
中科院分区：
文献类型：
--
作者：
Baehr, Ariane;Singer, Anna;Muenk, Carsten
泡沫病毒是一种在灵长类和非灵长类动物中广泛分布的逆转录病毒。我们在这里用猿猴和非猿猴来源的衣壳检测FV对β-干扰素(干扰素-β)的敏感性。我们的数据显示，在人HOS和THP-1感染的早期，干扰素-β对Fv有明显的抑制作用，但对HEK293T细胞没有明显的抑制作用。FV的进入后限制不是由干扰素诱导的MXB蛋白介导的，MXB蛋白最近被鉴定为整合前针对人类免疫缺陷病毒(HIV)的衣壳相互作用限制因子。无论是MXA或MXB在HEK293T细胞中的异位表达，还是在CRISPR/CAS MXB THP-1基因敲除细胞中不表达MXB，都不影响被试FV的感染。干扰素-β处理的THP-1和THP-1 KO MXB细胞对Fv的抑制程度相同。总之，这些数据表明，干扰素-β在感染早期抑制FV，MXB不是FV的限制因素。(C)爱思唯尔公司出版的2015年。
Foamy viruses (FV) are retroviruses that are widely distributed in primate and non-primate animal species. We tested here FV with capsids of simian and non-simian origin for sensitivity to interferon-beta (IFN-beta). Our data show significant inhibition of FV by IFN-beta early in infection of human HOS and THP-1 but not of HEK293T cells. The post-entry restriction of FV was not mediated by the interferon-induced MxB protein that was recently identified as a capsid-interacting restriction factor targeting Human immunodeficiency virus (HIV) before integration. Neither the ectopic expression of MxA or MxB in HEK293T cells nor the lack of MxB expression in CRISPR/CAS MxB THP-1 knockout cells impacted the infection of the tested FV. IFN-beta treated THP-1 and THP-1 KO MxB cells showed the same extend of restriction to FV. Together, the data demonstrate that IFN-beta inhibits FV early in infection and that MxB is not a restriction factor of FV. (C) 2015 Published by Elsevier Inc.