Capture of uropathogenic E. coli by using synthetic glycan ligands specific for the pap-pilus.
Capture of uropathogenic E. coli by using synthetic glycan ligands specific for the pap-pilus.
复制标题
使用乳头菌毛特异性的合成聚糖配体捕获尿路致病性大肠杆菌。
DOI：
10.1002/cbic.201200582
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Iyer,SuriS
中科院分区：
文献类型：
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作者：
Yosief,HailemichaelO;Weiss,AlisonA;Iyer,SuriS
Biotinylated mono‐ and biantennary di‐/trisaccharides were synthesized to evaluate their ability to captureE. colistrains that express pilus types with different receptor specificities. The synthesized biotinylated di‐/trisaccharides contain Galα(1→4)Gal, Galα(1→4)GalNHAc, GalNHAcα(1→4)Gal, Galα(1→4)Galβ(1→4)Glc and GalNHAcα(1→4)Galβ(1→4)Glc as carbohydrate epitopes. These biotinylated oligosaccharides were immobilized on streptavidin‐coated magnetic beads, and incubated with different strains of liveE. coli. Capturing ability was assessed by using a luciferase assay that detects bacterial ATP. The trisaccharides containing Galα(1→4)Galβ(1→4)Glc and the disaccharides containing Galα(1→4)Gal as the epitopes exhibited strong capturing ability for uropathogenicE. colistrains with thepappilus genotype, including CFT073, J96 and J96 pilE. The same ligands failed to captureE. colistrains withfim,prs, orfocgenotypes. Uropathogenic CFT073 was also captured moderately by biantennary disaccharides containing a GalNHAc moiety at the reducing end; however, other saccharides containing GalNHAc at the nonreducing end did not capture the CFT073 strain. These synthetic glycoconjugates could potentially be adapted as rapid diagnostic agents to differentiate between differentE. colipathovars.
