High-speed multiparameter photophysical analyses of fluorophore libraries.
High-speed multiparameter photophysical analyses of fluorophore libraries.
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DOI：
10.1021/acs.analchem.5b00607
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发表时间：
2015
影响因子：
7.4
通讯作者：
Jimenez R
中科院分区：
文献类型：
--
作者：
Dean KM;Davis LM;Lubbeck JL;Manna P;Friis P;Palmer AE;Jimenez R
There is a critical need for high-speed multi-parameter photophysical measurements of large libraries of fluorescent probe variants for imaging and biosensor development. We present a microfluidic flow cytometer that rapidly assays 104–105 member cell-based fluorophore libraries, simultaneously measuring fluorescence lifetime and photo-bleaching. Together, these photophysical characteristics determine imaging performance. We demonstrate the ability to resolve the diverse photophysical characteristics of different library types and the ability to identify rare populations.
