THE EXTRACTION AND CHARACTERIZATION OF HUMAN NAIL KERATIN
THE EXTRACTION AND CHARACTERIZATION OF HUMAN NAIL KERATIN
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DOI：
10.1016/0923-1811(91)90003-g
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发表时间：
1991-01-01
影响因子：
4.6
通讯作者：
OGAWA H
中科院分区：
文献类型：
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作者：
KITAHARA T;OGAWA H
我们调查了指甲角蛋白通过连续提取与还原剂浓度增加（50 mM和200 mM 2-巯基乙醇），并检查每个提取的角蛋白凝胶电泳，然后通过免疫印迹。结果发现指甲中含有表皮角蛋白和毛发角蛋白。用50 mM 2-巯基乙醇，只能提取表皮类型的角蛋白，包括皮肤分化特异性角蛋白，而从指甲中提取毛发类型的角蛋白需要200 mM 2-巯基乙醇。这些结果可能表明，指甲具有独特的性质，就其角蛋白组成。指甲中表皮角蛋白和毛发角蛋白对2-巯基乙醇的耐久性的差异表明，尽管在指甲中共存，但由表皮角蛋白多肽或毛发角蛋白多肽组成的两种不同类型的角蛋白丝可能具有不同的结构特征。
We investigated nail keratins obtained by sequential extraction with increasing reducing agent concentrations (50 mM and 200 mM 2-mercaptoethanol) and examined each of the extracted keratins by gel electrophoresis followed by immunoblotting. It was found that nail contained epidermal and hair keratins. With 50 mM 2-mercaptoethanol, only epidermal type of keratins including skin differentiation specific-keratin could be extracted, whereas hair type of keratins required 200 mM 2-mercaptoethanol for extraction from nail. These results may indicate that nail has unique properties with respect to its keratin compositions. The difference in durabilities of epidermal and hair keratins in nail against 2-mercaptoethanol suggests that two different types of the keratin filaments which are composed of epidermal or hair keratin polypeptides may have differing structural features, despite of co-existence in nail.