Direct assembly of gold nanoparticle "shells" on polyurethane microsphere "cores" and their application as enzyme immobilization templates
Direct assembly of gold nanoparticle "shells" on polyurethane microsphere "cores" and their application as enzyme immobilization templates
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DOI：
10.1021/cm020784a
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发表时间：
2003-05-20
影响因子：
8.6
通讯作者：
Sastry, M
中科院分区：
文献类型：
--
作者：
Phadtare, S;Kumar, A;Sastry, M
研究了水相金纳米粒子在聚亚安酯(PU)微球表面的组装，形成了[金纳米粒壳]-[聚氨酯核]结构。金纳米粒子在聚合物微球上的组装是通过聚合物中的氮基与纳米粒子的相互作用进行的。这种直接组装消除了对聚合物微球进行额外表面修饰的需要，这是其他基于聚合物的核壳结构协议中的重要一步。然后，将纳米金-PU材料与胃酶偶联，形成一类新的生物催化剂。相对于溶液中的游离酶，新的生物偶联材料显示出略高的生物催化活性，并显著提高了pH和温度稳定性。在胃酶生物偶联中使用金纳米颗粒标记的聚合物微球可以很容易地从反应介质中分离出来，并在六个反应周期内重复使用生物偶联物。
The assembly of aqueous gold nanoparticles on the surface of polyurethane (PU) spheres leading to [gold nanoparticle shell] - [polyurethane core] structures is demonstrated. The assembly of gold nanoparticles on the polymer microspheres occurs through interaction of the nitrogens in the polymer with the nanoparticles. Such direct assembly obviates the need to perform additional surface modification: of the polymer microspheres, which is an important step in other polymer-based core-shell structure protocols. The nanogold-PU material is then conjugated with the enzyme pepsin, leading to the formation of a new class of biocatalyst. In relation to the free enzyme in solution, the new bioconjugate material exhibited a slightly higher biocatalytic activity and significantly enhanced pH and temperature stability. The use of gold nanoparticle-labeled polymer microspheres in pepsin bioconjugation enables easy separation from the reaction medium and reuse of the bioconjugate over six reaction cycles.