Genes and novel sRNAs involved in PAHs degradation in marine bacteria Rhodococcus sp. P14 revealed by the genome and transcriptome analysis
Genes and novel sRNAs involved in PAHs degradation in marine bacteria Rhodococcus sp. P14 revealed by the genome and transcriptome analysis
复制标题
海洋细菌红球菌中参与 PAH 降解的基因和新型 sRNA。
DOI：
10.1007/s13205-020-2133-6
复制
发表时间：
2020-02-26
期刊：
影响因子：
2.8
通讯作者：
Hu, Zhong
中科院分区：
文献类型：
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作者：
Peng, Tao;Kan, Jie;Hu, Zhong
红球菌属P14能够降解多种多环芳烃(PAHs)。本研究在其基因组中鉴定了6个环羟化双加氧酶和24个与多环芳烃降解相关的单加氧酶基因。此外，还鉴定了丝氨酸水解酶、水合酶、乙醇脱氢酶、原儿茶酸3，4-双加氧酶、β-酮己二酸辅酶A转移酶和β-酮己基辅酶A硫解酶等与多环芳烃降解有关的基因。在基因组分析的基础上，在P14菌株中构建了多环芳烃的降解途径，结果表明多环芳烃被降解为乙酰辅酶A和琥珀酰辅酶A，然后通过TCA循环矿化为二氧化碳。此外，根据转录组数据，细胞色素P450(RS16725；RS16695；RS12220)、过氧化氢酶(RS15825)、脱氢酶(RS15755；RS18420)和水解酶(RS16460；RS24665)在多环芳烃降解过程中表达水平增加。此外，还鉴定了12个可能对多环芳烃降解具有调控功能的新的sRNA。本研究对多环芳烃在红球菌中的降解途径进行了展望。第14页。此外，首次证明了sRNAs在多环芳烃降解过程中可能具有调节功能。
Rhodococcus sp. P14 is able to degrade various polycyclic aromatic hydrocarbons (PAHs). In this study, 6 ring-hydroxylating dioxygenases and 24 monooxygenases genes related to PAHs degradation were identified in its genome. Moreover, various genes, like serine hydrolase, hydratase, alcohol dehydrogenase, protocatechuate 3,4-dioxygenase, β-ketoadipate CoA transferase and β-Ketoadipyl CoA thiolase, which were supposed to be involved in PAHs degradation were also identified. Based on the genome analysis, the proposed PAHs degradation pathway was constructed in P14 strain, which showed that PAHs was degraded into the acetyl CoA and succinyl CoA, then mineralized to CO 2 via the TCA cycle. Furthermore, several genes, including cytochrome P450 (RS16725; RS16695; RS12220), catalase (RS15825), dehydrogenase (RS15755; RS18420) and hydrolase (RS16460; RS24665), showed increased expression level during PAHs degradation according to the transcriptome data. In addition, 12 novel sRNAs which were supposed to have the regulation function in PAHs degradation were identified. This study gives us the outlook of PAHs degradation pathway in Rhodococcus sp. P14. Moreover, it first demonstrates that sRNAs may harbor the regulation function in PAHs degradation.