ETHANOL ALTERS ASTROCYTE DEVELOPMENT - A STUDY OF CRITICAL PERIODS USING PRIMARY CULTURES
ETHANOL ALTERS ASTROCYTE DEVELOPMENT - A STUDY OF CRITICAL PERIODS USING PRIMARY CULTURES
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DOI：
10.1007/bf00966217
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发表时间：
1990-05-01
影响因子：
4.4
通讯作者：
RENAUPIQUERAS, J
中科院分区：
文献类型：
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作者：
GUERRI, C;SAEZ, R;RENAUPIQUERAS, J
我们使用从 21 天大的大鼠胎儿中获得的星形胶质细胞进行原代培养，分析了产前饮酒对其发育过程中星形胶质细胞 DNA 和蛋白质合成的影响。还评估了星形胶质细胞在增殖和成熟期间对“体外”乙醇敏感性的变化。对照星形胶质细胞分别在第 8 天和第 15 天出现 DNA 和蛋白质合成峰值。研究发现，产前接触乙醇的胎儿的星形胶质细胞的 DNA 和蛋白质合成均显着减少。当对照星形胶质细胞在整个培养期间“体外”暴露于乙醇 (100mM) 时，也观察到了这种对 DNA 合成的影响。还评估了在增殖或分化期间短期（48小时）暴露于乙醇对星形胶质细胞对上述参数和细胞周期的影响以及从这些影响中可能恢复的影响。这两个时期都发现 DNA 和蛋白质合成减少。然而，在增殖期暴露于乙醇的星形胶质细胞中，DNA 合成和含量受到的影响更大。这种效应与细胞周期 G0/G1 期细胞的积累相关。另一方面，当暴露于乙醇的细胞在无酒精培养基中培养以评估恢复情况时，只有在第4至6天暴露于乙醇的细胞在第21天仍表现出DNA乙醇诱导的效果。总之，我们的结果表明，妊娠期间乙醇消耗会对皮质星形胶质细胞祖细胞造成严重损害。我们的结果进一步表明，尽管星形胶质细胞在增殖期间对酒精的毒性作用更敏感，但在神经胶质成熟期间接触乙醇也会改变其正常发育。
Using astrocytes obtained from 21-day-old rat fetuses, in primary culture, we have analyzed the effect of prenatal alcohol consumption on DNA and protein synthesis of astrocytes during their development. The variation in sensitivity of astrocytes to ethanol "in vitro" during the proliferation and maturation periods was also assessed. Control astrocytes showed peaks of DNA and protein synthesis at 8 and 15 days, respectively. A significant decrease in both DNA and protein synthesis was found in astrocytes from fetuses prenatally exposed to ethanol. This effect on DNA synthesis was also observed when control astrocytes were exposed to ethanol (100mM) "in vitro" during the entire culture period. The effects on astrocytes of short term (48h) exposure to ethanol during the proliferation or differentiation periods on the above mentioned parameters and on the cell cycle as well as the possible recovery from these effects were also evaluated. Decreases in DNA and protein synthesis were found in both periods. However, DNA synthesis and content were more affected in astrocytes exposed to ethanol during the proliferation period. This effect correlates with an accumulation of cells in the G0/G1 phase of the cell cycle. On the other hand, when cells exposed to ethanol were cultured in alcohol-free medium to assess recovery, only cells exposed to ethanol during days 4 to 6 still showed DNA ethanol-induced effects at 21 days. In conclusion, our results show that ethanol consumption during gestation induces serious damage to cortical astrocyte progenitor cells. Our results further demonstrate that although astrocytes are more sensitive to the toxic effect of alcohol during the proliferation period, exposure to ethanol during glial maturation also alters their normal development.