Comparing DNA quantity and quality using saliva collection following food and beverage consumption
Comparing DNA quantity and quality using saliva collection following food and beverage consumption
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DOI：
10.1186/s13104-019-4211-6
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发表时间：
2019-03-23
影响因子：
1.8
通讯作者：
Chapleau, Richard R.
中科院分区：
文献类型：
--
作者：
Hughes, Summer R.;Chapleau, Richard R.
目的：随着基因检测的民主化，研究人员，临床医生和教育工作者在收集基因分析样本时必须考虑不同程度的现场条件。对于基因分型或测序研究，研究设计者有多种选择，包括颊拭子和唾液采样。唾液收集的一个重要好处是，它可以远程完成，在一个人的家里的隐私。同样的好处也增加了合规风险。因此，我们这项研究的目的是看看唾液DNA收集试剂盒收集唾液的质量和数量是否会受到不遵循制造商指示的影响，即，结果：我们要求五名参与者根据制造商的指导以及在食用五种食物或饮料后收集唾液样本。我们使用光谱学、电泳和聚合酶链反应基因分型评估了纯化后的DNA数量和质量。与我们的假设一致，我们没有看到分离的DNA在数量或质量上的差异。从我们的研究结果，我们得出结论，制造商的说明书作为一个理想的指导方针，但收集设备是足够强大的，允许在家里或在现场采样的灵活性。
Objective: With the democratization of genetic testing, researchers, clinicians, and educators must consider the varying degree of field conditions when collecting samples for genetic analyses. For genotyping or sequencing studies, study designers have multiple options from which to choose, including cheek swabs and saliva sampling. One significant benefit of saliva collection is that it can be done remotely, in the privacy of one's home. This same benefit adds a risk of compliance. Therefore, our goal with this study was to see if the quality and quantity of the saliva collection by a saliva DNA collection kit would be affected by not following the manufacturer's directions, i.e., drinking or eating right before collection.Results: We asked five participants to collect saliva samples according to the manufacturer's guidance and also after consuming five food items or beverages. We evaluated DNA quantity and quality post-purification using spectroscopy, electrophoresis, and polymerase chain reaction genotyping. Consistent with our hypothesis, we did not see a difference in quantity or quality of the isolated DNA. From our results, we conclude that the manufacturer's instructions serve as an ideal guideline, but the collection devices are robust enough to permit flexibility in sampling at home or in the field.