Evidence of natural Wolbachia infections in field populations of Anopheles gambiae.
Evidence of natural Wolbachia infections in field populations of Anopheles gambiae.
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DOI：
10.1038/ncomms4985
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发表时间：
2014-06-06
影响因子：
16.6
通讯作者：
Catteruccia F
中科院分区：
文献类型：
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作者：
Baldini F;Segata N;Pompon J;Marcenac P;Shaw WR;Dabiré RK;Diabaté A;Levashina EA;Catteruccia F
沃尔巴克氏体是母体传播的细胞内细菌，通过操纵昆虫的繁殖和免疫力侵入昆虫种群，从而限制了许多人类病原体的传播。实验性的沃尔巴克氏体感染可以减少实验室中按蚊体内的疟原虫数量，然而，野外按蚊的自然沃尔巴克氏体感染从未有过报道。在这里，我们展示了西非布基纳法索冈比亚按蚊中沃尔巴克氏体感染的证据。16 S rRNA基因的测序鉴定了两个季节中雌性和雄性生殖系中的沃尔巴克氏体序列，并确定这些序列从母亲垂直传递给后代。阳性样本的全基因组测序表明，在An. gambiae属于一种新的沃尔巴克氏体菌株，与感染其他节肢动物的菌株相关但不同。自然按蚊种群中沃尔巴克氏体感染的证据促进了对可能使用自然沃尔巴克氏体-按蚊协会来限制疟疾传播的进一步调查。 沃尔巴克氏体细菌生活在许多昆虫物种的细胞内，操纵宿主的繁殖和免疫反应。在这里，作者表明这些微生物也感染了传播疟疾的按蚊的野生种群，支持Wolbachia限制疟疾传播的潜在用途。
Wolbachia are maternally transmitted intracellular bacteria that invade insect populations by manipulating their reproduction and immunity and thus limiting the spread of numerous human pathogens. Experimental Wolbachia infections can reduce Plasmodium numbers in Anopheles mosquitoes in the laboratory, however, natural Wolbachia infections in field anophelines have never been reported. Here we show evidence of Wolbachia infections in Anopheles gambiae in Burkina Faso, West Africa. Sequencing of the 16S rRNA gene identified Wolbachia sequences in both female and male germlines across two seasons, and determined that these sequences are vertically transmitted from mother to offspring. Whole-genome sequencing of positive samples suggests that the genetic material identified in An. gambiae belongs to a novel Wolbachia strain, related to but distinct from strains infecting other arthropods. The evidence of Wolbachia infections in natural Anopheles populations promotes further investigations on the possible use of natural Wolbachia–Anopheles associations to limit malaria transmission. Wolbachia bacteria live within the cells of many insect species, manipulating their hosts’ reproduction and immune responses. Here, the authors show that these microbes also infect wild populations of malaria-spreading Anopheles mosquitoes, supporting a potential use of Wolbachia to limit malaria transmission.