Expression of forkhead box transcription factor genes Foxp1 and Foxp2 during jaw development.
Expression of forkhead box transcription factor genes Foxp1 and Foxp2 during jaw development.
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DOI：
10.1016/j.gep.2016.03.001
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Jeong J
中科院分区：
文献类型：
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作者：
Cesario JM;Almaidhan AA;Jeong J
面部的发育受大量基因的调控，这些基因在时间和空间上都有特定的模式表达。虽然在确定面部口腔区域的基因特征方面已经取得了重大进展，但那些调节流产(侧面)区域发育的基因在很大程度上仍不清楚。最近，我们发现口腔发育的关键调控因子LIM Homeobox(LHX)6和LHX8抑制了口腔区编码叉头盒转录因子Foxp1和Foxp2的基因的表达。为了深入了解FoxP基因在面部区域特异性发育中的潜在作用，我们以高空间和时间分辨率研究了它们在小鼠胚胎第一咽弓(颌骨的原基)中的表达模式。Foxp1和Foxp2主要表达于第一咽弓的前部和后部，包括发育中的颞下颌关节。通过双免疫荧光和双荧光RNA原位杂交，我们发现Foxp1在肌肉、骨和结缔组织的祖细胞中都有表达。Foxp2在骨和结缔组织祖细胞亚群中表达，但在成肌细胞中不表达。这两种基因都不在牙间充质中表达，也不在经历了广泛生长和形态发生的腭架的口腔半部中表达。我们一起首次证明了Foxp1和Foxp2在颅面发育过程中表达。我们的数据表明，FoxP基因可能调节颌骨的流产和后部的发育。
Development of the face is regulated by a large number of genes that are expressed in temporally and spatially specific patterns. While significant progress has been made on characterizing the genes that operate in the oral region of the face, those regulating development of the aboral (lateral) region remain largely unknown. Recently, we discovered that transcription factors LIM homeobox (LHX) 6 and LHX8, which are key regulators of oral development, repressed the expression of the genes encoding forkhead box transcription factors, Foxp1 and Foxp2, in the oral region. To gain insights into the potential role of the Foxp genes in region-specific development of the face, we examined their expression patterns in the first pharyngeal arch (primordium for the jaw) of mouse embryos at a high spatial and temporal resolution. Foxp1 and Foxp2 were preferentially expressed in the aboral and posterior parts of the first pharyngeal arch, including the developing temporomandibular joint. Through double immunofluorescence and double fluorescent RNA in situ hybridization, we found that Foxp1 was expressed in the progenitor cells for the muscle, bone, and connective tissue. Foxp2 was expressed in subsets of bone and connective tissue progenitors but not in the myoblasts. Neither gene was expressed in the dental mesenchyme nor in the oral half of the palatal shelf undergoing extensive growth and morphogenesis. Together, we demonstrated for the first time that Foxp1 and Foxp2 are expressed during craniofacial development. Our data suggest that the Foxp genes may regulate development of the aboral and posterior regions of the jaw.