Thin-layer chromatography of green leaves extracts: Zinc migrates into pheophytin on TLC silica plates with fluorescence indicator (F254)
Thin-layer chromatography of green leaves extracts: Zinc migrates into pheophytin on TLC silica plates with fluorescence indicator (F254)
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绿叶提取物的薄层色谱：在带有荧光指示剂 (F254) 的薄层色谱硅胶板上，锌迁移到脱镁叶绿素中
DOI：
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发表时间：
2016
影响因子：
1.6
通讯作者：
R. Schmid
中科院分区：
文献类型：
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作者：
B. J. Sjursnes;L. Kvittingen;R. Schmid
在旨在测试不同流动相以进行分离的实验中，这些板保存了几天以供比较[3]。然后观察到，在含有F254的TLC板上，叶绿素的颜色从灰色变为绿色，而叶绿素a保持绿色，与不含F254的TLC板上的情况相反。据我们所知，这方面从未在教科书或一般的层析书籍中讨论过，例如[4-6]，或在关于薄层层析的具体文献中[7-14]。然而，关于添加到TLC板上的荧光指示剂F254的问题已有报道[15]。Saitoh等人。报道了F254对硅胶60薄层层析中合成的叶绿素衍生物的干扰影响，怀疑F254中的锌离子参与了[16]。
During the experiments aimed to test different mobile phases for separation, the plates were kept several days for comparison [3]. It was then observed that, on TLC plates with F254, the color of pheophytin changed from grey to green and that chlorophyll a remained green, as opposed to on TLC plates without F254. This aspect was, to our knowledge, never discussed in textbooks or general chromatography books, e.g., [4–6], or in the specific literature on thin-layer chromatography [7–14]. Problems with the fluorescent indicator, F254, added to TLC plates have, however, been reported [15]. Saitoh et al. reported disturbing influences of F254 on synthetic pheophytin derivatives in TLC on silica gel 60, suspecting that zinc ions in F254 were involved [16].