Drosophila signal peptide peptidase is an essential protease for larval development
Drosophila signal peptide peptidase is an essential protease for larval development
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DOI：
10.1534/genetics.104.039933
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发表时间：
2005-05-01
期刊：
影响因子：
3.3
通讯作者：
Kornberg, TB
中科院分区：
文献类型：
--
作者：
Casso, DJ;Tanda, S;Kornberg, TB
我们确定了果蝇信号肽肽酶基因（Spp），编码一个多通道跨膜蛋白酶。果蝇SPP与人信号肽肽酶（SPP）同源，与早老素有较远的亲缘关系。我们发现，像人类SPP，果蝇SPP可以蛋白水解模型信号肽，是敏感的SPP蛋白酶抑制剂，它定位于内质网。果蝇SPP的表达首先在胚带延伸处明显，并且在晚期胚胎中，其在唾液腺、腺胃和气管中是稳健的。携带保守残基突变或携带Spp基因缺陷的果蝇有缺陷的气管，并在幼虫时死亡。
We identified the Drosophila melanogaster Signal peptide peptidase gene (Spp) that encodes a multipass transmembrane aspartyl protease. Drosophila SPP is homologous to the human signal peptide peptidase (SPP) and is distantly related to the presenilins. We show that, like human SPP, Drosophila SPP can proteolyze a model signal peptide and is sensitive to an SPP protease inhibitor and that it localizes to the endoplasmic reticulum. Expression of Drosophila SPP was first apparent at germ band extension, and in late embryos it was robust in the salivary glands, proventriculus, and tracheae. Flies bearing mutations in conserved residues or carrying deficiencies for the Spp gene had defective tracheae and died as larvae.