Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat
Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat
复制标题
杂交小麦扩增片段长度多态性标记遗传多样性分析
DOI：
10.4238/2015.august.7.2
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发表时间：
2015
影响因子：
0.4
通讯作者：
翁群珠
中科院分区：
文献类型：
--
作者：
M.Ejaz;朱启迪;张改生;牛娜;赵惠燕;翁群珠
利用扩增片段长度多态性（Amplified fragment length polymorphism，AFLP）标记对10个小麦雄性不育系（异源胞质同核）和1个恢复系的遗传多样性进行了分析。利用64对扩增片段长度多态性引物组合对这些不育系进行了评价，其中13对引物产生了多态性条带，共产生682条片段。在682个片段中，有113个是多态性的。多态性信息含量和标记指数值证明了本研究中使用的引物组合的实用性。对基因型数据进行算术平均和主坐标分析的非加权对组法揭示了基于遗传关系的种质聚类，并将种质与其恢复系分为2类。Jaccard相似系数表明不育系间具有较好的遗传多样性，表明不育系在育种中具有一定的实用价值;分子方差分析表明，群体内变异较大，占总变异的77%，群体间比较占总变异的23%，差异显著。在这项研究中观察到的分子多样性将是有用的，选择合适的加入植物改良和杂交通过分子育种方法和制定合适的保护策略。
Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study. Unweighted pair group method with arithmetic mean and principal coordinate analysis of the genotypic data revealed clustering of accessions based on genetic relationships, and accessions were separated into 2 groups with their restorer line. Jaccard’s similarity coefficient values suggested good variability among the male sterile lines, indicating their utility in breeding programs.The fallouts of analysis of molecular variance showed large within-group population variation, accounting for 77% of variation, while among-group comparison accounted for 23% of the total molecular variation, which was statistically significant. The molecular diversity observed in this study will be useful for selecting appropriate accessions for plant improvement and hybridization through molecular-breeding approaches and for developing suitable conservation strategies.