A pilot study of the role of the claustrum in attention and seizures in rats
A pilot study of the role of the claustrum in attention and seizures in rats
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DOI：
10.1016/j.eplepsyres.2018.01.006
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发表时间：
2018-02-01
影响因子：
2.2
通讯作者：
Koubeissi, Mohamad Z.
中科院分区：
文献类型：
--
作者：
Bayat, Arezou;Joshi, Sweta;Koubeissi, Mohamad Z.
目的：屏状核与意识有关，癫痫持续状态患者的 MRI 显示屏状核信号强度增加。为了研究屏状核在认知和癫痫发作中的作用，我们（1）评估了屏状核高频刺激（HFS）对操作室表现的影响； （2）通过脑脑诱发电位（CCEP）研究幽闭海马和幽闭海马的连通性； (3)研究屏状核在红藻氨酸诱导的(KA)癫痫发作中的作用。方法：对成年雄性Sprague-Dawley大鼠进行操作性条件反射训练，并在双侧屏状核和海马中植入电极。 Claustrum HFS (50 Hz) 以双边和单边的方式进行，强度从 50 mu A 增加到 1000 mu A，并评估表现分数。 CCEP 的研究方法是对屏状核双极刺激（0.1 Hz 的 1 毫秒宽脉冲）的响应进行平均。通过视频脑电图记录分析 KA 癫痫发作。结果：广义估计方程分析显示，相对于休息时间，幽闭刺激降低了任务绩效得分（双侧：-15.8 个百分点，p < 0.0001；单侧：-15.2，p < 0.0001）。在一些刺激下，老鼠表现出刺激锁定的注意力下降，有时甚至无法完成操作任务。 CCEP 展示了幽闭海马和幽闭海马的连通性。一些 KA 癫痫发作似乎源自屏状核。结论：操作性条件反射任务的发现表明，刺激屏状核可以改变注意力或意识。 CCEP 证明了两个屏状体之间以及屏状体和海马体之间的连通性。这种连接可能是边缘系统癫痫发作时意识改变的回路的一部分。最后，KA 癫痫发作显示屏状核早期受累，这一发现也支持屏状核在伴随认知障碍性癫痫发作的意识改变中可能发挥的作用。
Objective: The claustrum has been implicated in consciousness, and MRIs of patients with status epilepticus have shown increased claustral signal intensity. In an attempt to investigate the role of claustrum in cognition and seizures, we (1) assessed the effect of high-frequency stimulation (HFS) of the claustrum on performance in the operant chamber; (2) studied interclaustral and claustrohippocampal connectivity through cerebro-cerebral evoked potentials (CCEPs); and (3) investigated the role of claustrum in kainate-induced (KA) seizures.Methods: Adult male Sprague-Dawley rats were trained in operant conditioning and implanted with electrodes in bilateral claustra and hippocampi. Claustrum HFS (50 Hz) was delivered bilaterally and unilaterally with increasing intensities from 50 to 1000 mu A, and performance scores were assessed. CCEPs were studied by averaging the responses to bipolar stimulations, 1-ms wide pulses at 0.1 Hz to the claustrum. KA seizures were analyzed on video-EEG recordings.Results: Generalized Estimating Equations analysis revealed that claustral stimulation reduced task performance scores relative to rest sessions (bilateral: -15.8 percentage points, p < 0.0001; unilateral: -15.2, p < 0.0001). With some stimulations, the rats showed a stimulus-locked decrease in attentiveness and, occasionally, an inability to complete the operant task. CCEPs demonstrated interclaustral and claustrohippocampal connectivity. Some KA seizures appeared to originate from the claustrum.Conclusions: Findings from the operant conditioning task suggest stimulation of the claustrum can alter attention or awareness. CCEPs demonstrated connectivity between the two claustra and between the claustrum and the hippocampi. Such connectivity may be part of the circuitry that underlies the alteration of awareness in limbic seizures. Lastly, KA seizures showed early involvement of the claustrum, a finding that also supports a possible role of the claustrum in the alteration of consciousness that accompanies dyscognitive seizures.