Bioavailability of Gold Nanomaterials to Plants: Importance of Particle Size and Surface Coating
Bioavailability of Gold Nanomaterials to Plants: Importance of Particle Size and Surface Coating
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DOI：
10.1021/es3019397
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发表时间：
2012-08-07
影响因子：
11.4
通讯作者：
Bertsch, Paul M.
中科院分区：
文献类型：
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作者：
Judy, Jonathan D.;Unrine, Jason M.;Bertsch, Paul M.
我们使用模式生物烟草（Nicotiana tabacum L。cv Xanthi（烟草）和小麦（小麦）来研究植物对涂覆有鞣酸盐（T-MNM）或柠檬酸盐（C-MNM）的直径为10-、30-和50-nm的Au人造纳米材料（MNM）的吸收。采用透射电子显微镜（TEM）、动态光散射（DLS）和电泳迁移率测量分别表征初级粒径、流体动力学粒径和zeta电位。对于小麦和烟草，分别将植物水培暴露于NP 3或7天。使用电感耦合等离子体质谱法（ICP-MS）测定体积平均Au浓度。使用激光烧蚀ICP-MS（LA-ICP-MS）和扫描X射线荧光显微镜（mu XRF）测定组织样品中Au的空间分布。C-MNM和T-MNM在烟草中均有生物积累，但在小麦中均未观察到生物积累。这些结果表明，MNM的大小和不同的表面化学性质的范围很广的生物可利用的植物，提供机械信息的细胞壁孔在植物吸收的MNM的作用，并提出问题的重要性，植物物种的MNM生物积累。
We used the model organisms Nicotiana tabacum L. cv Xanthi (tobacco) and Triticum aestivum (wheat) to investigate plant uptake of 10-, 30-, and 50-nm diameter Au manufactured nanomaterials (MNMs) coated with either tannate (T-MNMs) or citrate (C-MNMs). Primary particle size, hydrodynamic size, and zeta potential were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), and electrophoretic mobility measurements, respectively. Plants were exposed to NPs hydroponically for 3 or 7 days for wheat and tobacco, respectively. Volume averaged Au concentrations were determined using inductively coupled plasma mass spectrometry (ICP-MS). Spatial distribution of Au in tissue samples was determined using laser ablation ICP-MS (LA-ICP-MS) and scanning X-ray fluorescence microscopy (mu XRF). Both C-MNMs and T-MNMs of each size treatment bioaccumulated in tobacco, but no bioaccumulation of MNMs was observed for any treatment in wheat. These results indicate that MNMs of a wide range of size and with different surface chemistries are bioavailable to plants, provide mechanistic information regarding the role of cell wall pores in plant uptake of MNMs, and raise questions about the importance of plant species to MNM bioaccumulation.