MOLECULAR ASSEMBLY OF PROTEINS AND CONJUGATED POLYMERS - TOWARD DEVELOPMENT OF BIOSENSORS
MOLECULAR ASSEMBLY OF PROTEINS AND CONJUGATED POLYMERS - TOWARD DEVELOPMENT OF BIOSENSORS
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DOI：
10.1002/bit.260450204
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发表时间：
1995-01-20
影响因子：
3.8
通讯作者：
KAPLAN, DL
中科院分区：
文献类型：
--
作者：
AYYAGARI, MS;PANDE, R;KAPLAN, DL
描述了一种分子组装体，其中共轭聚合物与光动力蛋白连接。这种用生物素功能化的共轭聚合物被设计成可以物理吸附在玻璃表面上或从玻璃表面化学生长。链霉亲和素衍生蛋白通过强生物素-链霉亲和素相互作用固定在生物素化聚合物基质上。该组件构建在光纤表面或玻璃毛细管内壁上，是用于检测有机磷杀虫剂等环境污染物的生物传感器的组成部分。系统中的蛋白质可以被任何感兴趣的生物大分子替代。我们研究了一种具体情况，即碱性磷酸酶的抑制。该酶催化反应产生化学发光的中间化合物，并监测化学发光信号以检测和定量杀虫剂，例如对氧磷和甲基对硫磷。初步结果表明，检测结果为 ppb 级，响应时间小于 1 分钟。 (C) 1995 约翰威利父子公司
A molecular assembly in which a conjugated polymer is interfaced with a photodynamic protein is described. The conjugated polymer, functionalized with biotin, is designed such that it can be physisorbed on or chemically grown off a glass surface. The streptavidin-derivatized protein is immobilized on the biotinylated polymer matrix through the strong biotin-streptavidin interactions. The assembly, built on the surface of an optical fiber or on the inside walls of a glass capillary, forms an integral part of a biosensor for the detection of environmental pollutants such as organophosphorus-based insecticides. The protein in the system can be replaced by any biological macromolecule of interest. We study one specific case, the inhibition of the enzyme alkaline phosphatase. The enzyme catalyzes a reaction producing an intermediate compound that chemiluminesces, and the chemiluminescence signal is monitored to detect and quantify insecticides such as paraoxon and methyl parathion. Preliminary results indicate ppb level detection with response time less than 1 minute. (C) 1995 John Wiley & Sons, Inc.