CD163 positive subsets of blood dendritic cells: The scavenging macrophage receptors CD163 and CD91 are coexpressed on human dendritic cells and monocytes
CD163 positive subsets of blood dendritic cells: The scavenging macrophage receptors CD163 and CD91 are coexpressed on human dendritic cells and monocytes
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DOI：
10.1016/j.imbio.2006.05.019
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发表时间：
2006-01-01
期刊：
影响因子：
2.8
通讯作者：
Moller, Bjarne Kuno
中科院分区：
文献类型：
--
作者：
Maniecki, Maciej Bogdan;Moller, Holger Jon;Moller, Bjarne Kuno
CD 163和CD 91是在单核细胞分化成抗炎巨噬细胞表型期间表达高度增加的清除受体。此外，CD91在单核细胞衍生的树突状细胞（MoDC）中表达，其中该受体被认为对于呈递在树突状细胞表面上用于T细胞刺激的CD91靶向抗原的内化是重要的。尽管它们在功能上重叠，但从未比较过CD91和CD163的表达，并且尚未表征单核细胞-树突状细胞谱系中CD163的表达。应用流式细胞术检测了31例健康献血员和15例HIV-1患者外周血及5例新生儿脐带血中树突状细胞（DC）CD163的表达。从MACS纯化的DCs中提取总RNA，用实时逆转录聚合酶链反应测定CD 163 mRNA。应用流式细胞仪检测糖皮质激素和佛波酯对单核细胞和树突状细胞CD163和CD91表达的影响。我们在人血液中鉴定了具有树突状细胞特征的两个CD163（+）亚群，CD1631（lo）和CD163 hi，它们共同构成了DC的重要部分。流式细胞术检测两个亚群均为[lin]-CD4（+）ILT（3）+HLA-DR（+）CD11c（+），CD163 mRNA在MACS纯化的人DC中可检测到。糖皮质激素上调DC上的CD163，佛波酯治疗显著降低表面表达。总体而言，与健康患者（10.5%[95%CI：8.0 - 12.5]）相比，HIV-1患者（19.3%[95%CI：14.7 - 26.3%]）中DC上的CD163表达显著增加，p <0.001。CD163（lo）亚群为CD16（+），而CD163（hi）亚群为CD16（-）。这两个亚群均为CD91（+），因此构成了最近描述的CD91（+）CD11c（+）树突状细胞亚群的亚部分。在人单核细胞上也证实了CD 163和CD 91的共表达，其在糖皮质激素处理后表现出CD 163和CD 91表达的增加。我们现在已经表明，CD163和CD91在人单核细胞上共表达和共调节。此外，已经鉴定了两个CD163（+）DC亚群，其构成最近描述的CD91（+）CD11c（+）树突状细胞亚群的一部分。CD163表达模式表明，如果抗原靶向CD163，则它们可诱导类似于靶向CD91的抗原的免疫刺激应答。(c)2006年Elsevier GmbH。All rights reserved.
CD 163 and CD91 are scavenging receptors with highly increased expression during the differentiation of monocytes into the anti-inflammatory macrophage phenotype. In addition, CD91 is expressed in monocyte-derived dendritic cells (MoDCs), where the receptor is suggested to be important for internalization of CD91-targeted antigens to be presented on the dendritic cell surface for T-cell stimulation. Despite their overlap in functionality, the expression of CD91 and CD163 has never been compared and the expression of CD163 in the monocyte-dendritic cell lineage is not yet characterized. CD163 expression in dendritic cells (DCs) was investigated using multicolor flow cytometry in peripheral blood from 31 healthy donors and 15 HIV-1 patients in addition to umbilical cord blood from 5 newborn infants. Total RNA was isolated from MACS purified DCs and CD163 mRNA was determined with real-time reverse transcriptase polymerase chain reaction. The effect of glucocorticoid and phorbol ester stimulation on monocyte and dendritic cell CD163 and CD91 expression was investigated in cell culture of mononuclear cells using multicolor flow cytometry. We identified two CD163(+) subsets in human blood with dendritic cell characteristics, CD1631(lo) and CD163 hi, together constituting a substantial fraction of DCs. Both subsets were characterized as [lin]-CD4(+)ILT(3)+HLA-DR(+)CD11c(+) by flow cytometry, and CD163 mRNA was readily detectable in MACS purified human DCs. CD163 on DCs was upregulated by glucocorticoid, and treatment by phorbol ester signiticantly decreased surface expression. Overall, the expression of CD163 on DCs was significantly increased in HIV-1 patients (19.3% [95% CI: 14.7-26.3%]) compared to healthy patients (10.5% [95% CI: 8.0-12.5]) p < 0.001. The CD163(lo) subset was CD16(+), whereas the CD163(hi) subset was CD16(-). Both subsets were CD91(+), thereby constituting a subfraction of the recently described CD91(+)CD11c(+) dendritic cell subset. Coexpression of CD163 and CD91 was also demonstrated on human monocytes, which upon glucocorticoid treatment exhibited an increase in both CD163 and CD91 expression. We have now shown that CD163 and CD91 are coexpressed and coregulated on human monocytes. In addition, two subsets of CD163(+) DCs constituting a fraction of the recently described CD91(+)CD11c(+) dendritic cell subset have been identified. The CD163 expression pattern suggests that if antigens are targeted to CD163 they may induce an immunostimulatory response like that of CD91-targeted antigens. (c) 2006 Elsevier GmbH. All rights reserved.