Terpenoids are Widespread in Actinomycetes: A Correlation of Secondary Metabolism and Genome Data
Terpenoids are Widespread in Actinomycetes: A Correlation of Secondary Metabolism and Genome Data
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DOI：
10.1002/cbic.201100641
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发表时间：
2012-01-23
期刊：
影响因子：
3.2
通讯作者：
Dickschat, Jeroen S.
中科院分区：
文献类型：
--
作者：
Citron, Christian A.;Gleitzmann, Julia;Dickschat, Jeroen S.
所有细菌的基因组与公开可用的测序基因组已被筛选的倍半萜环化酶同源物的存在下，导致在55个推定的土臭素环化酶，23个同源的2-甲基异黄酮环化酶，和98个其他倍半萜环化酶同源物的鉴定。到目前为止，这些酶中的大多数都是在放线菌中发现的。采用闭环溶出法从31株放线菌和4株其它类群的35株细菌中分离出萜类化合物，并通过GC-MS分析鉴定了其结构。鉴定的挥发性萜类化合物根据结构相似性和它们的生物合成关系进行分组，并且这些分析的结果与可用的基因组信息相关，从而对细菌萜类化合物的生物合成产生有价值的新见解。
The genomes of all bacteria with publicly available sequenced genomes have been screened for the presence of sesquiterpene cyclase homologues, resulting in the identification of 55 putative geosmin synthases, 23 homologues of 2-methylisoborneol synthases, and 98 other sesquiterpene cyclase homologues. Most of these enzymes by far were found in actinomycetes. The terpenoid volatiles from 35 strains, including 31 actinomycetes and four strains from other taxa, were collected by using a closed-loop stripping apparatus and identified by GC-MS. All of these bacteria apart from one strain encode sesquiterpene cyclase homologues in their genomes. The identified volatile terpenoids were grouped according to structural similarities and their biosynthetic relationship, and the results of these analyses were correlated to the available genome information, resulting in valuable new insights into bacterial terpene biosynthesis.