Culture of mesothelial cells from bovine pericardium and characterization of their arachidonate metabolism.
Culture of mesothelial cells from bovine pericardium and characterization of their arachidonate metabolism.
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牛心包间皮细胞的培养及其花生四烯酸代谢的表征。
DOI：
10.1016/0167-4889(87)90001-2
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Prescott,SM
中科院分区：
文献类型：
--
作者：
Satoh,K;Prescott,SM
建立了牛心包间皮细胞的培养体系，并对其花生四烯酸代谢进行了研究。培养的细胞的鉴定是基于形态学观察，并通过电泳分析的细胞骨架蛋白，这表明了模式以前报道的mesothelium细胞。因子VIII相关抗原存在间接免疫荧光，但细胞没有血栓调节蛋白活性。培养的心包细胞将花生四烯酸代谢为6-酮前列腺素F1α和少量前列腺素E2。完整的心包壁层碎片产生了相同的代谢物，但去除间皮素的碎片则没有。培养的间皮细胞产生94.6 ± 60.4（平均值± S.D.）外源性花生四烯酸为117.3 ± 13.6 ng/mg（n = 3），缓激肽为18.3 ± 11.3 ng/mg（n= 5），组胺为8.4 ± 4.3 ng/kg（n= 4），凝血酶为11.2 ± 9.7 ng/mg（n= 5）。所有这些值均显著高于（P< 0.05）对照组（2.1 ± 1 ng/mg，n= 5）。从这些结果，我们得出结论，间皮细胞占花生四烯酸代谢的心包。前列腺素I2的产生是对生理或病理激动剂的反应，并且是大量的。也就是说，它与内皮细胞大致相同。间皮细胞释放类花生酸进入心包腔可能在心脏生理学和病理学中具有重要作用。
Cultures of mesothelial cells from bovine pericardium were established and their arachidonate metabolism was characterized. The identification of the cultured cells was based on morphological observations, and by electrophoretic analysis of cytoskeletal proteins, which demonstrated a pattern previously reported for mesothelial cells. Factor VIII-related antigen was present by indirect immunofluorescence, but the cells had no thrombomodulin activity. The cultured pericardial cells metabolized arachidonic acid to 6-ketoprostaglandin F1αand a small amount of prostaglandin E2. The same metabolites were produced by pieces of intact parietal pericardium but not by pieces from which mesothelium had been removed. The cultured mesothelial cells produced 94.6 ± 60.4 (mean ± S.D.) ng/mg (n= 3) cell protein of 6-ketoprostaglandin F1αin response to the calcium ionophore A23187, 117.3 ± 13.6 ng/mg (n= 3) with exogenous arachidonic acid, 18.3 ± 11.3 ng/mg (n= 5) with bradykinin, 8.4 ± 4.3 ng/kg (n= 4) with histamine and 11.2 ± 9.7 ng/mg (n= 5) with thrombin. All of these values were significantly higher (P< 0.05) than the control (2.1 ± 1 ng/mg,n= 5). From these results, we conclude that the mesothelial cells account for the arachidonate metabolism in the pericardium. The production of prostaglandin I2occurs in response to physiological or pathological, agonists, and is substantial. That is, it is approximately the same as endothelial cells. The release of eicosanoids by mesothelial cells into the pericardial space may have a significant role in cardiac physiology and pathology.