High yield micronodular cirrhosis in the rat.
High yield micronodular cirrhosis in the rat.
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DOI：
10.1016/s0016-5085(82)80126-1
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发表时间：
1982-12
期刊：
影响因子：
29.4
通讯作者：
E. Proctor;Krisna Chatamra
中科院分区：
文献类型：
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作者：
E. Proctor;Krisna Chatamra
尽管本世纪大部分时间都在研究严重失代偿性小结节性肝硬化，但仍没有可靠的模型可以预测其合理数量的产生。目前最成功的模型是在苯巴比妥诱导的大鼠中吸入四氯化碳蒸汽，其严重肝硬化的发生率低，死亡率高，因为没有办法确定对四氯化碳的反应变化和维持恒定的临界肝损伤水平。本文提出了一种解决这一老问题的新方法，即在轻度氟烷/氧气麻醉下，通过大鼠对灌胃四氯化碳的每日体重变化来监测临界损伤的变化和水平；每次的反应被用来校准随后的四氯化碳剂量，以适应单个大鼠。该方法可使约75%的大鼠在给药8-10次四氯化碳后产生肝硬化伴腹水。
Although studied for most of this century there is still no reliable model of severe decompensated micronodular cirrhosis that can be predictably produced in reasonable quantity. The current most successful model, inhalation of carbon tetrachloride vapor in the phenobarbitone-induced rat, has a low yield of severe cirrhosis and a high death rate because there is no way to determine both the variation in response to carbon tetrachloride and the maintenance of a constant critical level of liver damage. A new approach to this old problem is described in which both variation and level of critical damage are monitored by the daily weight change of the rat in response to intragastric carbon tetrachloride given during light halothane/oxygen anesthesia; the response each time being used to calibrate the subsequent dose of carbon tetrachloride to fit the individual rat. The method is effective in producing cirrhosis with ascites in about 75% of rats after 8–10 doses of carbon tetrachloride.