Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375
Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375
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DOI：
10.1007/s00122-005-0116-y
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发表时间：
2005-12-01
影响因子：
5.4
通讯作者：
Monforte, AJ
中科院分区：
文献类型：
--
作者：
Eduardo, I;Arús, P;Monforte, AJ
甜瓜的双单倍体系 (DHL) 群体源自韩国品种“Songwhan Charmi”登记号 PI161375 (SC)（包括在园艺组 conomon 中）与西班牙品种“Piel de Sapo”（PS）（包括在园艺组 inodorus 中）之间的杂交，用于开发一系列近等基因系 (NIL)。这些亲本系代表了非常不同的甜瓜品种组，在果实、植物、疾病反应和分子水平上具有重要差异。该杂交是甜瓜种质资源中多态性最强的杂交之一。选定的 DHL 与 PS 回交，并进行进一步的回交和自交，使用覆盖甜瓜遗传图谱的分子标记监测 SC 的基因渗入。获得了 57 个 NIL 的最终集合，其中包含 PS 遗传背景中来自 SC 的独特独立渐渗。该集合内的基因渗入覆盖了至少 85% 的 SC 基因组，平均基因渗入大小为 41 cM，相当于 SC 基因组的 3.4%。绘制基因或数量性状基因座的平均分辨率为 18.90 cM。这套 NIL 是一个潜在的强大工具，可用于研究涉及甜瓜果实品质和其他重要复杂性状的数量性状基因座，以及在来自外来来源的现代品种中引入新的遗传变异。 NIL 还可用作甜瓜育种项目中的竞争前育种系。
A doubled haploid line (DHL) population of melon derived from a cross between the Korean cultivar "Songwhan Charmi" accession PI161375 (SC), included in the horticultural group conomon, and the Spanish cultivar "Piel de Sapo" (PS), included in the horticultural group inodorus, was used to develop a collection of near isogenic lines (NILs). These parental lines represent very different melon cultivar groups, with important differences at fruit, plant, disease response and molecular level. This cross is one of the most polymorphic ones within melon germplasm. Selected DHLs were backcrossed to PS and further backcrossing and selfing was performed, monitoring introgressions from SC using molecular markers covering the melon genetic map. A final collection of 57 NILs was obtained, containing a unique independent introgression from SC in the PS genetic background. The introgressions within the collection cover at least 85% of the SC genome with an average introgression size of 41 cM, corresponding to 3.4% of the SC genome. The average resolution for mapping genes or quantitative trait loci is 18.90 cM. This set of NILs is a potentially powerful tool for the study of quantitative trait locus involved in melon fruit quality and other important complex traits, and the introduction of new genetic variability in modern cultivars from exotic sources. The NILs can also be used as pre-competitive breeding lines in melon breeding projects.