EXPRESSION OF J-CHAIN RNA IN CELL-LINES REPRESENTING DIFFERENT STAGES OF LYMPHOCYTE-B DIFFERENTIATION
EXPRESSION OF J-CHAIN RNA IN CELL-LINES REPRESENTING DIFFERENT STAGES OF LYMPHOCYTE-B DIFFERENTIATION
复制标题
DOI：
10.1016/0092-8674(81)90132-x
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
KOSHLAND, ME
中科院分区：
文献类型：
--
作者：
MATHER, EL;ALT, FW;KOSHLAND, ME
在B细胞分化为分泌五聚体IgM的过程中，启动了五聚体连接成分J链的合成。通过分析B细胞分化不同阶段的小鼠[肿瘤]细胞系，探讨J链合成的启动机制。通过无细胞翻译和J链产物的特异性免疫沉淀来评价功能性J链mRNA的表达。用c[互补]DNA分子克隆获得的J链探针杂交检测前体mRNA的表达。在代表未分化B淋巴细胞的淋巴瘤系中未发现J链特异性RNA。在代表igm分泌浆细胞的杂交细胞系中鉴定出以下3种J链RNA：成熟信息约1.5 kb[千碱基]和2个次要成分，分别为2.5和0.92 kb。因此，B细胞与抗原或有丝分裂原相遇必须触发影响J链序列转录或其核内稳定的事件。
During B cell differentiation to pentamer IgM secretion, synthesis of the pentamer joining component, the J chain, is initiated. The mechanism for initiating J chain synthesis was studied by analyzing murine [neoplastic] cell lines representing different stages in B cell differentiation. The expression of functional J chain mRNA was evaluated by cell-free translation and specific immunoprecipitation of a J chain product. The expression of precursor mRNA was examined by hybridization with a J chain probe obtained by molecular cloning of c[complementary]DNA. No J chain-specific RNA was demonstrated in a lymphoma line representative of an undifferentiated B lymphocyte. The following 3 species of J chain RNA were identified in hybrid cell lines representative of IgM-secreting plasma cells: a mature message of approximately 1.5 kb [kilobase] and 2 minor components of 2.5 and 0.92 kb. The encounter of a B cell with antigen or mitogen must therefore trigger events that effect transcription of J chain sequences or their intranuclear stabilization.