Heterogeneity of serum low density lipoproteins in normal human subjects.
Heterogeneity of serum low density lipoproteins in normal human subjects.
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DOI：
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发表时间：
1981-02
影响因子：
6.5
通讯作者：
M. M. Shen-M.;R. Krauss;F. Lindgren;T. Forte
中科院分区：
文献类型：
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作者：
M. M. Shen-M.;R. Krauss;F. Lindgren;T. Forte
用平衡密度梯度超离心法分离了12名健康人血清低密度脂蛋白（LDL）的6个亚组分，平均密度为1.0268 ~ 1.0597g/ml。平均校正的峰值浮选率（Sof）测定的分析ultracenthagation，和平均粒径测定的负染色电子显微镜，都显着下降，随着密度的增加的亚组分。的亚馏分的化学组成的主要差异，注意到，包括显着降低甘油三酯含量和胆固醇酯甘油三酯的比例较高的中间馏分相比，那些最高和最低密度。组分2浓度与HDL呈正相关（P <0.01），与VLDL呈负相关（P <0.001），组分4浓度与HDL呈负相关（P <0.05），与VLDL（P <0.001）和IDL（P <0.01）呈正相关。因此，LDL可能包括不同结构和组成的亚种，这些亚种也可能具有不同的代谢和致动脉粥样硬化作用。
Equilibrium density gradient ultracentrifugation of serum low density lipoprotein (LDL) from twelve healthy human subjects was used to separate six subfractions with mean density ranging from 1.0268 to 1.0597 g/ml. Mean corrected peak flotation rate (Sof) measured by analytic ultracentrifugation, and mean particle diameter determined by negative staining electron microscopy, both declined significantly with increasing density of the subfractions. Major differences in chemical composition of the subfractions were noted, including a significantly lower triglyceride content and higher ratio of cholesteryl ester to triglyceride in the middle fractions compared with those of highest and lowest density. Concentration of fraction 2 correlated positively with HDL (P less than 0.01) and negatively with VLDL (P less than 0.001); concentration of fraction 4 correlated negatively with HDL (P less than 0.05) and positively with VLDL (P less than 0.001) and IDL (P less than 0.01). LDL may thus include subspecies of differing structure and composition which might also have different metabolic and atherogenic roles.