Effect of UVA/Riboflavin Collagen Crosslinking on Biomechanics of Artificially Swollen Corneas
Effect of UVA/Riboflavin Collagen Crosslinking on Biomechanics of Artificially Swollen Corneas
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DOI：
10.1167/iovs.17-22814
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发表时间：
2018-02-01
影响因子：
4.4
通讯作者：
Jayaram, Sandeep M.
中科院分区：
文献类型：
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作者：
Hatami-Marbini, Hamed;Jayaram, Sandeep M.
目的。本研究的目的是表征 UVA/核黄素胶原交联治疗的角膜水合和硬化效果之间的关系，并研究在此治疗程序之前人工肿胀角膜如何影响拉伸性能的改善。方法。使用多种低渗和等渗核黄素溶液在不同水合水平下对猪角膜进行胶原交联。交联前样品的厚度被视为其水合作用的代表，并用于将它们分为不同的厚度组。使用动态机械分析（DMA）机进行机械拉伸测试。机械测试期间样品的水合作用与它们交联时的水合作用保持相似。记录的力用于计算胶原交联处理之前作为厚度（水合）函数的最大拉伸应力和切线模量。 结果。胶原蛋白与低渗或等渗溶液的交联显着增加了角膜拉伸模量（P < 0.05）。与在较低水合条件下交联的角膜相比，在交联前肿胀的角膜表现出明显更软的拉伸性能（P < 0.05）。虽然拉伸性能的改善程度取决于水化程度，但当在机械实验中水化程度保持相似时，在较高水化情况下交联的样品的刚度与在较低水化情况下交联的样品的刚度没有显着差异。结论。如果在相似的水合条件下测量拉伸性能，在胶原交联处理之前将猪角膜肿胀到不同程度不会显着改变生物力学的改善量。
PURPOSE. The purpose of this study was to characterize the relation between corneal hydration and stiffening effects of the UVA/riboflavin collagen crosslinking treatment and to investigate how artificially swelling the cornea prior to this treatment procedure affects tensile property improvement.METHODS. Porcine corneas were collagen crosslinked in vitro at different hydration levels using a number of hypoosmolar and isoosmolar riboflavin solutions. Thickness of the specimens prior to crosslinking was taken as a proxy for their hydration and was used to divide them into different thickness groups. A Dynamic Mechanical Analysis (DMA) machine was used to perform mechanical tensile tests. The hydration of specimens during the mechanical tests was kept similar to the hydration at which they were crosslinked. The recorded force was used to calculate the maximum tensile stress and tangent modulus as a function of thickness (hydration) prior to collagen crosslinking treatment.RESULTS. Collagen crosslinking with either a hypoosmolar or isoosmolar solution significantly increased corneal tensile modulus (P < 0.05). Corneas that were swollen prior to crosslinking showed significantly softer tensile properties compared with those that were crosslinked at lower hydration (P < 0.05). Although the degree of tensile property improvement was hydration dependent, the stiffness of samples crosslinked at higher hydration was not significantly different than the stiffness of those crosslinked at lower hydration when the hydration was kept similar in the mechanical experiments.CONCLUSIONS. Swelling porcine corneas to the different extents prior to collagen crosslinking treatment does not significantly change the amount of biomechanical improvement if tensile properties are measured at similar hydration.