Phosphatidylcholine/siphingomyelin metabolism crosstalk inside the nucleus
Phosphatidylcholine/siphingomyelin metabolism crosstalk inside the nucleus
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DOI：
10.1042/bj20070758
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发表时间：
2008-03-01
影响因子：
4.1
通讯作者：
Magni, Mariapia Viola
中科院分区：
文献类型：
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作者：
Albi, Elisabetta;Lazzarini, Remo;Magni, Mariapia Viola
已知磷脂代表染色质的次要组分。近年来研究发现，由于存在与其代谢相关的酶，如中性鞘磷脂酶、鞘磷脂合酶、反向鞘磷脂合酶和磷脂酰胆碱特异性磷脂酶C，这些脂质直接在细胞核内代谢。染色质酶活性在细胞增殖、分化和/或凋亡期间变化，独立于存在于核膜、微粒体或细胞膜中的酶活性。本研究的目的是研究脂质代谢的细胞核膜和染色质的串扰从大鼠肝脏在体外和体内分离。中性鞘磷脂酶活性对磷脂酰胆碱特异性磷脂酶C和鞘磷脂合酶的影响，这丰富了核内二酰基甘油池，和磷脂酰胆碱特异性磷脂酶C活性对中性鞘磷脂酶和反向鞘磷脂合酶的影响，这丰富了核内神经酰胺池，进行了研究。结果表明，在染色质中，存在磷脂酰胆碱/鞘磷脂代谢串扰，调节核内神经酰胺/甘油二酯库。D 609对酶活性有明显的抑制作用，证明了这种串扰的特异性。染色质脂质代谢在体内细胞增殖过程中被激活，表明它可能在细胞功能中发挥作用。串扰的可能机制进行了讨论，考虑到该领域的最新进展。
It is known that phospholipids represent a minor component of chromatin. It has been highlighted recently that these lipids are metabolized directly inside the nucleus, thanks to the presence of enzymes related to their metabolism, Such as neutral sphingomyelinase, sphingomyelin synthase, reverse sphingomyelin synthase and phosphatidylcholine-specific phospholipase C. The chromatin enzymatic activities change during cell proliferation, differentiation and/or apoptosis, independently from the enzyme activities present in nuclear membrane, microsomes or cell membranes. This present study aimed to investigate crosstalk in lipid metabolism in nuclear membrane and chromatin isolated from rat liver in vitro and in vivo. The effect of neutral sphingomyelinase activity on phosphatidylcholine-specific phospholipase C and sphingomyelin synthase, which enrich the intranuclear diacylglycerol pool, and the effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, was investigated. The results show that in chromatin, there exists a phosphatidyicholine/sphingomyelin metabolism crosstalk which regulates the intranuclear ceramide/diacylglycerol pool. The enzyme activities were inhibited by D609, which demonstrated the specificity of this crosstalk. Chromatin lipid metabolism is activated in vivo during cell proliferation, indicating that it could play a role in cell function. The possible mechanism of crosstalk is discussed here, with consideration to recent advances in the field.