Characteristic Quantities of Corneal Epithelial Structures in Confocal Laser Scanning Microscopic Volume Data Sets
Characteristic Quantities of Corneal Epithelial Structures in Confocal Laser Scanning Microscopic Volume Data Sets
复制标题
共焦激光扫描显微体积数据集中角膜上皮结构的特征量
DOI：
10.1097/ico.0b013e31826247bf
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发表时间：
2013
期刊：
影响因子：
2.8
通讯作者：
O. Stachs
中科院分区：
文献类型：
--
作者：
R. Prakasam;K. Winter;Mario Schwiede;S. Allgeier;A. Zhivov;B. Köhler;R. Guthoff;O. Stachs
目的：全自动量化健康人角膜中不同上皮细胞层的形态特征。方法：对6名健康志愿者的单眼进行活体共聚焦激光扫描显微镜检查。使用层间距为0.4 m的160张图像（400 × 400 m）的堆栈来生成上皮的全厚度体积数据集。使用适当的图像分析算法定量基底（BC）和中间细胞（IC）层的尺寸和形状因子。评价参数包括平均面积、密实度、密实度、长短径和最大边界距离。结果：BC和IC的平均面积从80至160 m²呈线性增加。在大直径和小直径以及最大边界距离方面观察到类似的趋势。BC和IC的大直径在13.2和17.0米之间，而这些细胞的小直径在8.6和12.4米之间。BC和IC的最大边界距离为7.0 ~ 9.1m。上皮细胞的致密性聚集在1.45和1.5左右，而细胞坚固性测量值在1.0和1.03之间。结论：使用该方法可以在无需人工干预的情况下计算出几个特征形态量。我们的研究显示了有希望的结果，并表明，这种全自动形态定量可以成功地应用于评估正常和各种角膜疾病的上皮细胞的微观结构变化。
Purpose: Fully automated quantification of the morphologic features of different epithelial cell layers in healthy human corneas. Methods: In vivo confocal laser scanning microscopy was performed on the unilateral eyes of 6 healthy volunteers. Stacks of 160 images (400 × 400 &mgr;m) with an interslice distance of 0.4 &mgr;m were used to generate full thickness volume data sets of the epithelium. Size and shape factors of basal (BC) and intermediate cell (IC) layers were quantified using appropriate image analysis algorithms. Evaluated parameters include mean area, compactness, solidity, major and minor diameter, and maximum boundary distance. Results: Mean area of BC and IC demonstrated a linear increase from 80 to 160 &mgr;m². A similar trend was noted with major and minor diameter and maximum boundary distance. Major diameters of BC and IC measured between 13.2 and 17.0 &mgr;m, whereas minor diameter of these cells measured between 8.6 and 12.4 &mgr;m. The maximum boundary distance of BC and IC ranged from 7.0 to 9.1 &mgr;m. Compactness of epithelial cells clustered around 1.45 and 1.5, whereas cell solidity measured between 1.0 and 1.03. Conclusion: Several characteristic morphologic quantities can be calculated using this methodology without manual intervention. Our study demonstrated promising results and suggests that this fully automated morphologic quantification can be successfully applied to assess microstructural changes of the epithelium in normal and various corneal disorders.