Menin interacts with ss-catenin in osteoblast differentiation
Menin interacts with ss-catenin in osteoblast differentiation
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Menin 与 ss-catenin 在成骨细胞分化中相互作用
DOI：
10.1055/s-0030-1270527
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发表时间：
2011
期刊：
影响因子：
2.2
通讯作者：
Kaji H
中科院分区：
文献类型：
--
作者：
Inoue Y;Hendy GN;Canaff L;Seino S;Kaji H
Menin通过与BMP-2信号分子Smad 1/5和Runx 2相互作用，促进多能间充质干细胞向成骨细胞谱系的承诺。然而，menin与骨中Wnt-β-catenin通路之间的关系尚不清楚。通过转染menin反义构建体减少menin表达并不改变小鼠间充质C2 C12和成骨细胞MC 3 T3-E1中β-连环蛋白的水平。然而，在C2 C12和MC 3 T3-E1细胞中，menin与β-catenin以及LEF-1共免疫沉淀。在C2 C12和MC 3 T3-E1细胞中，通过反义menin转染减少menin表达拮抗β-连环蛋白诱导的pGL 3-OT荧光素酶报告基因构建体的转录活性。反义menin转染拮抗BMP-2和β-catenin刺激的C2 C12细胞中Runx 2和碱性磷酸酶水平的增加。数据显示，menin与β-catenin在小鼠间充质细胞和成骨细胞中相互作用，并且表明这种相互作用对于成骨细胞分化是重要的。
Menin promotes the commitment of pluripotent mesenchymal stem cells to the osteoblast lineage by interacting with the BMP-2 signaling molecules Smad1/5, and Runx2. However, the relationship between menin and the Wnt-β-catenin pathway in bone is unclear. Reduction of menin expression by transfection of a menin antisense construct did not alter the levels of β-catenin in mouse mesenchymal C2C12 and osteoblastic MC3T3-E1 cells. However, menin co-immunoprecipitated with β-catenin as well as LEF-1 in C2C12 and MC3T3-E1 cells. Reduction of menin expression by antisense menin transfection antagonized β-catenin-induced transcriptional activity of the pGL3-OT luciferase reporter construct in C2C12 and MC3T3-E1 cells. Antisense menin transfection antagonized the BMP-2 and β-catenin-stimulated increases in Runx2 and alkaline phosphatase levels in C2C12 cells. The data show that menin interacts with β-catenin in mouse mesenchymal and osteoblastic cells, and suggest that the interaction is important for osteoblast differentiation.