Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells.
Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells.
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DOI：
10.1038/srep23808
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发表时间：
2016-04-01
影响因子：
4.6
通讯作者：
Wakayama T
中科院分区：
文献类型：
--
作者：
Mizutani E;Torikai K;Wakayama S;Nagatomo H;Ohinata Y;Kishigami S;Wakayama T
通过细胞核移植克隆动物为保护濒危哺乳动物物种提供了机会。然而，存在与从身体收集供体细胞相关的风险，例如动物的意外伤害或死亡。在这里，我们报告生产的克隆小鼠从尿来源的细胞收集非侵入性。大多数的尿源性细胞存活下来，并可作为核移植的供体，无需任何预处理。核移植后，38-77%的重构胚发育至桑椹胚/囊胚，其中内细胞团和滋养外胚层的细胞数与对照组相似。雄性和雌性克隆小鼠是从移植到受体雌性的克隆胚胎中产生的，这些克隆动物在相互交配时生长到成年并自然分娩幼崽。结果表明，这些克隆小鼠具有正常的生育能力。在另外的实验中，26个核移植胚胎干细胞系是从108个克隆胚泡中建立的，这些胚泡来自于四个小鼠品系，包括近交系和F1杂种，具有相对较高的成功率。因此，可以非侵入性地收集来自尿的细胞，其可以通过使用核移植而用于拯救濒危哺乳动物物种，而不会对动物造成伤害。
Cloning animals by nuclear transfer provides the opportunity to preserve endangered mammalian species. However, there are risks associated with the collection of donor cells from the body such as accidental injury to or death of the animal. Here, we report the production of cloned mice from urine-derived cells collected noninvasively. Most of the urine-derived cells survived and were available as donors for nuclear transfer without any pretreatment. After nuclear transfer, 38–77% of the reconstructed embryos developed to the morula/blastocyst, in which the cell numbers in the inner cell mass and trophectoderm were similar to those of controls. Male and female cloned mice were delivered from cloned embryos transferred to recipient females, and these cloned animals grew to adulthood and delivered pups naturally when mated with each other. The results suggest that these cloned mice had normal fertility. In additional experiments, 26 nuclear transfer embryonic stem cell lines were established from 108 cloned blastocysts derived from four mouse strains including inbreds and F1 hybrids with relatively high success rates. Thus, cells derived from urine, which can be collected noninvasively, may be used in the rescue of endangered mammalian species by using nuclear transfer without causing injury to the animal.