Role for the Burkholderia pseudomallei capsular polysaccharide encoded by the wcb operon in acute disseminated melioidosis.
Role for the Burkholderia pseudomallei capsular polysaccharide encoded by the wcb operon in acute disseminated melioidosis.
复制标题
由 wcb 操纵子编码的类鼻疽伯克霍尔德氏菌荚膜多糖在急性播散性类鼻疽中的作用。
DOI：
10.1128/iai.00824-09
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发表时间：
2009
影响因子：
3.1
通讯作者：
Gherardini,FrankC
中科院分区：
文献类型：
--
作者：
Warawa,JonathanM;Long,Dan;Rosenke,Rebecca;Gardner,Don;Gherardini,FrankC
The capsular polysaccharide ofBurkholderia pseudomalleiis an essential virulence determinant that is required for protection from host serum cidal activity and opsonophagocytosis. In this study, the immune response directed against aB. pseudomalleicapsule mutant (JW270) was investigated in an acute respiratory murine model. JW270 was significantly attenuated in this model (∼2 logs) to levels resembling those of avirulentBurkholderia thailandensis. At lethal doses, JW270 colonized the lung, liver, and spleen at levels similar to the wild-type strain levels and was found to trigger reduced pathology in the liver and spleen. Several cytokine responses were altered in these tissues, and importantly, the levels of gamma interferon were reduced in the livers and spleens of JW270-infected mice but not in the lungs. These results suggest that the capsular polysaccharide ofB. pseudomalleiis a critical virulence determinant in respiratory tract infections and that it is an important antigen for generating the Th1 immune response commonly observed in systemic melioidosis. Furthermore, the data suggest that host recognition ofB. pseudomalleicapsular polysaccharide in the lungs may not be as important to the disease outcome as the innate immune response in the peripheral organs.
