2,7-dichlorofluorescin oxidation and reactive oxygen species: What does it measure?
2,7-dichlorofluorescin oxidation and reactive oxygen species: What does it measure?
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DOI：
10.1006/cbir.2000.0556
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发表时间：
2000-01-01
影响因子：
3.9
通讯作者：
Bartosz, G
中科院分区：
文献类型：
--
作者：
Jakubowski, W;Bartosz, G
细胞内2，7-二氯荧光素(H_2DCF)氧化常被用来测量细胞内活性氧物种(ROS)的产生。H_2DCF的氧化速率取决于谷胱甘肽的浓度，谷胱甘肽是ROS的替代靶标。我们的结果表明，由于各种原因，包括抗氧化剂的耗尽，H2DCF氧化速率的增加被解释为一般氧化应激的指示，而不是ROS形成增加的特定证据。(C)2000年学术出版社。
Intracellular 2,7-dichlorofluorescin (H2DCF) oxidation is often used to measure the production of reactive oxygen species (ROS) within cells. The rate of H2DCF oxidation depends on the concentration of glutathione, which is an alternative target for ROS. Our results suggest that increased rate of H2DCF oxidation be interpreted as an indication of general oxidative stress due to a variety of reasons, including depletion of antioxidants, rather than as a specific proof of augmented ROS formation. (C) 2000 Academic Press.