Generation of induced pluripotent stem cell line (ZZUi019-A) derived from skin fibroblasts from a healthy volunteer
Generation of induced pluripotent stem cell line (ZZUi019-A) derived from skin fibroblasts from a healthy volunteer
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源自健康志愿者皮肤成纤维细胞的诱导多能干细胞系 (ZZUi019-A) 的产生
DOI：
10.1016/j.scr.2021.102285
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发表时间：
2021
影响因子：
1.2
通讯作者：
Xu Yuming
中科院分区：
文献类型：
--
作者：
Wang Yanlin;Sun Huifang;Wang Zhuoya;Zhang Qi;Yang Jing;Liu Yutao;Liu Han;Shi Changhe;Wang Yanlin;Xu Yuming
神经系统疾病的研究受到难以从人类获得神经细胞的限制。诱导性多能干细胞（induced pluripotent stem cells，iPSCs）技术的发展和实验体系的建立为神经细胞的体外定向诱导分化提供了可行的方法。在该项目中，我们招募了一名50岁的汉族男性作为健康志愿者，并使用非整合重编程方法成功构建了他的皮肤成纤维细胞来源的iPSC。同时进行了相关的多能性鉴定实验。该细胞系将在后续实验中纳入正常对照组，为神经系统疾病的研究提供重要参考。
The research on nervous system diseases is limited by the difficulty in obtaining nerve cells from humans. The development of induced pluripotent stem cells (iPSCs) technology and the experimental system provide a feasible method for directional induced differentiation of nerve cellsin vitro. In this project, we recruited a 50-year-old Han Chinese man as a healthy volunteer, and successfully constructed his skin fibroblast-derived iPSCs using the non-integrated reprogramming method. At the same time, the related pluripotency identification experiments were completed. This cell line will be included in the normal control group in follow-up experiments, providing an important reference for the study of neurological diseases.