Further Characterization of Foliar Flavonoids in Crossostephium chinense and their Geographic Variation
Further Characterization of Foliar Flavonoids in Crossostephium chinense and their Geographic Variation
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DOI：
10.1177/1934578x1400900207
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发表时间：
2014-02
影响因子：
1.8
通讯作者：
Ayumi Uehara;J. Kitajima;G. Kokubugata;T. Iwashina
中科院分区：
文献类型：
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作者：
Ayumi Uehara;J. Kitajima;G. Kokubugata;T. Iwashina
对产自日本和台湾的中国贯众叶中的黄酮类化合物进行了分离和鉴定。十八种黄酮苷元、毛地黄黄酮、芹菜素、毛蕊花素、黄菊醇、5，7，4 ′-三羟基-6，3 ′，5 ′-三甲氧基黄酮、茉莉花苷、西芹素、槲皮素3-甲醚、阿昔拉素、金脾醇-D、小蓟醇、阿朴美茨格尔苷、5，7，3 ′-三羟基-6，4 ′，5 ′-三甲氧基黄酮、毛地黄黄酮3′，4 ′-二甲醚、小蓟醇、泽兰素、荆芥素、5，7，3 ′，4 ′-四羟基-6，5 ′-二甲氧基黄酮，通过UV、1H和13 C NMR光谱、LC-MS和HPLC与真实样品进行比较来鉴定。这些化合物主要存在于叶片表面。另外还分离得到了四种黄酮苷类化合物，即槲皮素3，7-二-O-葡萄糖苷、槲皮素3-O-芸香糖苷、毛地黄素7-O-葡萄糖苷和芹菜素7-O-芸香糖苷。HPLC测定结果表明，不同采集点的黄酮类化合物含量存在差异。
Foliar flavonoids of Crossostephium chinense in Japan and Taiwan were isolated and further characterized. Eighteen flavonoid aglycones, luteolin, apigenin, hispidulin, chrysoeriol, 5,7,4′-trihydroxy-6,3′,5′-trimethoxyflavone, jaceosidin, cilsimaritin, quercetin 3-methyl ether, axillarin, chrysosplenol-D, cirsiliol, apometzgerin, 5,7,3′-trihydroxy-6,4′,5′-trimethoxyflavone, luteolin 3′,4′-dimethyl ether, cirsilineol, eupatilin, nepetin and 5,7,3′,4′-tetrahydroxy-6,5′-dimethoxyflavone, were identified by UV, 1H and 13C NMR spectroscopic, LC-MS and HPLC comparisons with authentic samples. The compounds existed on the leaf surface. Four flavonoid glycosides, quercetin 3,7-di-O-glucoside, quercetin 3-O-rutinoside, luteolin 7-O-glucoside and apigenin 7-O-rutinoside, were also isolated as the intracellular flavonoids. It was shown by HPLC survey that variation of the species’ flavonoids occurs among the collection sites.