Differential effects of the null alleles at the three Wx loci on the starch-pasting properties of wheat
Differential effects of the null alleles at the three Wx loci on the starch-pasting properties of wheat
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DOI：
10.1007/s001220051394
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Sawada, S
中科院分区：
文献类型：
--
作者：
Araki, E;Miura, H;Sawada, S
小麦淀粉的直链淀粉/支链淀粉比值和糊化特性对生产适销对路的面制品，特别是日本面条具有重要意义。为了确定三个Wx基因座的零突变是否导致淀粉糊化粘度的差异，我们分析了中国春与三个携带Wx零等位基因的单染色体替代系杂交产生的三组7A、4A和7D染色体重组替代系中与零突变相关的变异。3个Wx基因座上的零等位基因对淀粉糊化特性的影响存在差异。对于4A染色体，WX-B1B等位基因的作用是明确的，它提供了更高的峰值和破裂粘度。此外，在短臂着丝粒附近还检测到一个效应较小的QTL。Wx-A1蛋白的存在或不存在对峰值粘度和崩解粘度有一定的影响，但Wx-A1b的影响远小于Wx-B1b等位基因。由于缺失的Wx-D1b等位基因比野生型的Wx-D1a产生更高的粘度，因此可以检测到Wx-D1b基因座对崩解粘度的关联效应。虽然直链淀粉含量与崩解粘度之间的负相关在三个群体中普遍存在，但Wx基因座的零突变产生了一些与直链淀粉含量无关的变异。在三个重组群体中，崩解粘度的遗传变异比峰值粘度的遗传变异更明显。
The amylose/amylopectin ratio and the pasting properties of wheat starch are important in producing marketable flour products, especially Japanese noodles. To determine if null mutations at the three Wx loci confer differences in starch-pasting viscosity, we analyzed the variation associated with the null mutations in three separate sets of recombinant substitution lines of chromosomes 7A, 4A and 7D produced from crosses between Chinese Spring and three single-chromosome substitution lines carrying the null Wx alleles. Differential effects of null alleles at the three Wx loci on starch-pasting properties were revealed. With respect to chromosome 4A, the effect of the Wx-B1b allele, giving a higher peak and breakdown viscosity, was unambiguous. In addition, a QTL of minor effect was identified near the centromere on the short arm. The presence or absence of the Wx-A1 protein gave some variation in peak and breakdown viscosity, but the effects of Wx-A1b were much smaller than those of the Wx-B1b allele. Associated effects of the Wx-D1 locus were detected for the breakdown viscosity as the null Wx-D1b allele produced a higher viscosity than the wild-type Wx-D1a. While negative correlations between amylose content and breakdown viscosity were common in the three populations, the null mutations at the Wx loci produced some variation independent of amylose content. The genetic variation detected for breakdown viscosity was more evident than that for peak viscosity in all three recombinant populations.