VENTRAL ECTODERMAL RIDGE OF THE TAIL IN MOUSE EMBRYOS
VENTRAL ECTODERMAL RIDGE OF THE TAIL IN MOUSE EMBRYOS
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DOI：
10.1038/177787b0
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发表时间：
1956-01-01
期刊：
影响因子：
64.8
通讯作者：
GRUNEBERG, H
中科院分区：
文献类型：
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作者：
GRUNEBERG, H
哺乳动物和鸟类胚胎的肢芽显示出足板边缘的外胚层增厚，即所谓的顶端外胚层脊，并且已知该脊在肢的生长和分化中起重要作用1，2。最近发现，在10和11天大的小鼠胚胎中，在尾部的腹侧存在类似的外胚层增厚。在10日龄的胚胎中（顶臀长度为3.4-4.2 mm;透镜内陷仍然广泛开放），在腹侧出现一个非常明显的柱状上皮区，从尾尖向近端方向延伸约250 micra ;该区域的确切长度难以确定，因为它逐渐向近端消失。随附的显微照片（图1）是通过这种胚胎尾部的四个横切面（顶臀长4.1 mm;布因固定;苏木精和伊红; 7.5 μ m; × 125），间隔约68 μ m;（a）是这些切片的最远端，（d）是最近端。前三张切片上皮柱状带明显，第四张切片上皮柱状带消失。在11天龄的胚胎中（顶臀长5.3-5.7 mm），该结构相当不明显，并且在三个这样的胚胎中有一个胚胎中，它占据了稍微接近尾尖的位置。在12天大的胚胎中，这种结构几乎消失了。
THE limb buds of mammalian and avian embryos show a thickening of the ectoderm on the margin of the foot plates, the so-called apical ectodermal ridge, and it is known that this ridge plays an important part in the growth and differentiation of the limbs1,2. It has recently been discovered that a similar thickening of the ectoderm is present on the ventral side of the tail in 10- and 11-day old mouse embryos. In 10-day old embryos (crown-rump length 3.4–4.2 mm. ; lens invaginations still widely open), there occurs a very conspicuous zone of columnar epithelium on the ventral aspect which extends from the very tip of the tail in a proximal direction for roughly 250 micra ; the exact length of the zone is difficult to determine as it gradually disappears proximally. The accompanying photomicrographs (Fig. 1) are four transverse sections through the tail of such an embryo (crown-rump length 4.1 mm. ; Bouin fixation ; hæmatoxylin and eosin ; 7.5 micra ; × 125) at intervals of about 68 micra ; (a) is the most distal and (d) the most proximal of these sections. The columnar zone of epithelium is obvious in the first three sections but has disappeared in the fourth. In 11-day old embryos (crown-rump length 5.3–5.7 mm.), the structure is rather less conspicuous, and in one out of three such embryos it occupied a position somewhat proximal to the tail tip. In 12-day old embryos the structure has virtually disappeared.