Novel method for isolating mammalian cells defective in fluid-phase endocytosis.
Novel method for isolating mammalian cells defective in fluid-phase endocytosis.
复制标题
分离液相内吞作用缺陷的哺乳动物细胞的新方法。
DOI：
10.1007/bf01232650
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Draper,RK
中科院分区：
文献类型：
--
作者：
Wang,RH;Colbaugh,PA;Kuo,P;Bau,MY;Poppe,LM;Draper,RK
一种新的方法分离缺陷的突变体在液相内吞作用已经发展的基础上观察，内吞辣根过氧化物酶可以致命的细胞。该方法被用来从中国仓鼠卵巢细胞，被称为HRP-1，这是温度敏感的活力和辣根过氧化物酶内吞率在限制性温度降低了70%的突变体分离。在高温下，HRP-1细胞的分泌途径也有缺陷，其高尔基复合体在荧光显微镜下的分辨率消失。这些性质与先前描述的CHO细胞的两种突变体DS 28 -6和V.24.1相似。在互补试验中，突变体HRP-1、DS 28 -6和V.24.1似乎都在同一互补群中。
A new method for isolating mutants defective in fluid-phase endocytosis has been developed based on the observation that endocytosed horseradish peroxidase can be made lethal to cells. The method was used to isolate a mutant from Chinese hamster ovary cells, termed HRP-1, that was temperature-sensitive for viability and had a 70% reduction in the rate of horseradish peroxidase endocytosis at the restrictive temperature. At high temperature, HRP-1 cells were also defective in the secretory path and their Golgi complex disappeared at the resolution of fluorescence microscopy. These properties are similar to two previously described mutants of CHO cells, DS28-6 and V.24.1. In complementation tests, mutants HRP-1, DS28-6, and V.24.1 all appeared to be in the same complementation group.